Analytical equipment for detecting the concentration and chemical speciation of trace metals and reactive oxygen species in natural waters

海洋学界正在开展一项重大的国际方案(GEOTRACES http://www.geotraces.org/))，以研究微量元素和同位素的海洋生物地球化学循环，并阐明其分布中蕴含的海洋学信息。该计划包括对所有主要海洋盆地的详细水柱部分进行采样，并辅之以对具有战略位置的地区进行过程和时间序列研究，并将带来沉重的分析负担。该计划的规模在化学海洋学领域是史无前例的，有望在理解过去和现在的海洋过程及其在地球不断变化的气候系统中的作用方面取得巨大飞跃。例如，一些微量元素，如钴、铜、铁和锌，是必需的微量营养素。更好地了解控制这些可能限制生物的微量营养素的分布和形态的因素，将为了解它们在调节海洋生态系统的结构和生产力方面的作用、这些生态系统在封存二氧化碳方面的效率以及它们对气候变化的敏感性提供关键信息。该提案要求获得资金，以购置部件，组装流动注射分析/顺序注射分析系统，用于海水中生物活性痕量金属和活性氧类的化学发光、比色和荧光检测。这些分析方法已被证明是成本效益高、产量高、精度高的技术，可近实时测定海水中溶解的痕量金属，可以满足GEOTRACES等分析密集型项目的要求。

The oceanographic community is undertaking a major international program (GEOTRACES http://www.geotraces.org/) to study the marine biogeochemical cycling of trace elements and isotopes and to elucidate the oceanographic information embedded in their distribution. The program consists of sampling detailed water column sections in all major ocean basins, complemented by process and time-series studies in strategically located regions and will carry with it a heavy analytical burden. The scale of this program is unprecedented in the field of chemical oceanography and promises to provide a quantum leap forward in the understanding of ocean processes past and present and their role in Earth's changing climate system. For instance, some trace elements, such as Co, Cu, Fe, and Zn, are essential micronutrients. A better understanding of the factors controlling the distribution and speciation of these potentially biolimiting micronutrients will provide critical information for understanding their role in regulating the structure and productivity of marine ecosystems, the efficiency of these ecosystems at sequestering carbon dioxide and their sensitivity to climate change. This proposal requests funds to acquire components to assemble a flow injection analysis (FIA)/sequence injection analysis (SIA) system designed for the chemiluminescent, colorimetric and fluorometric detection of bioactive trace metals and reactive oxygen species in seawater. These analytical approaches have proven to be cost effective, high throughput, precise techniques for near real-time determinations of dissolved trace metals in seawater that can meet the requirements of analytically intense programs like GEOTRACES.