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Chemiluminescence and fluorescence imaging system for biochemical, molecular and cellular biology applications

用于生化、分子和细胞生物学应用的化学发光和荧光成像系统

基本信息

批准号：
390087-2010
负责人：
DeBruin, Lillian
金额：
$ 6.85万
依托单位：
Wilfrid Laurier University
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments - Category 1 (<$150,000)
财政年份：
2009
资助国家：
加拿大
起止时间：
2009-01-01 至 2010-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443103
关键词：
Chemiluminescence fluorescence imaging system biochemical

项目摘要

Researchers in the Departments of Biology and Chemistry at Wilfrid Laurier University (WLU) working in the fields of cell biology, biochemistry, and molecular biology will utilize the chemiluminscence and fluorescence imaging system to: study the effects of intracellular and extracellular protozoans on fish cell lines; characterize the various membrane microdomains in myelin; and determine the mechanism of protein targeting and import into chloroplasts. The requested imaging system will support the research programs of all three co-applicants (in addition to other current, and likely future researchers in both departments), and contribute to the training of undergraduate and graduate students. The imaging system includes a high-resolution ultra-cooled CCD camera with various excitation sources and emission filters. The system will permit visible, UV, fluorescence, and chemiluminescence applications for 1D and 2D gels, Western, Southern, and Northern blots, colony counting, organism/ tissue imaging, macroarrays, TLC plates, and microtiter plates. Therefore, the system will play a significant role in supporting and enhancing a broad range of research programs, and in so doing will help support newly established graduate programs in both departments, and thereby contribute to the growing research intensity within the Faculty of Science at WLU.

Wilfrid Laurier大学（WLU）生物学和化学系的研究人员在细胞生物学，生物化学和分子生物学领域工作，将利用荧光和荧光成像系统：研究细胞内和细胞外原生动物对鱼类细胞系的影响;表征髓鞘中的各种膜微区;并确定蛋白质靶向和导入叶绿体的机制。所要求的成像系统将支持所有三个共同申请人的研究计划（除了两个部门的其他当前和未来可能的研究人员），并有助于本科生和研究生的培训。成像系统包括一个高分辨率的超冷CCD相机与各种激发源和发射过滤器。该系统将允许用于1D和2D凝胶、Western、Southern和北方印迹、菌落计数、生物体/组织成像、宏阵列、TLC板和微量滴定板的可见光、UV、荧光和化学发光应用。因此，该系统将在支持和加强广泛的研究项目中发挥重要作用，这样做将有助于支持两个部门新设立的研究生课程，从而有助于WLU科学学院内不断增长的研究强度。