Immobilization, stabilization and biocatalysis of selected enzymatic systems in non-conventional media

非传统介质中选定酶系统的固定、稳定和生物催化

• 批准号: 36719-2010
• 负责人: Kermasha, Selim
• 金额: $ 2.19万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=450679
• 关键词: Immobilization; stabilization; biocatalysis; selected; enzymatic

The main objective of the applicant's research program is to advance and carry out experimental work that should contribute to the understanding of the mechanistic action and biocatalysis of selected immobilized enzymes, obtained from microbial sources, in non-conventional media, in particular organic solvent media (OSM). Although the research work carried out by the candidate addressed the immobilization and biocatalysis of lipoxygenase/hydroperoxide lyase system in OSM, the stability of these enzymes as well the elucidation of their mechanistic action in OSM are still among the major challenges to be overcome. The acquired knowledge oratory led us to move to novel horizons in research, in particular the use of nanotechnology approach for enzyme immobilization as well as modeling and ioinformatics for setting the experimental design and investigating the enzyme-substrate interaction.

申请人的研究计划的主要目标是推进和开展实验工作，这些工作应有助于理解从微生物来源获得的选定固定化酶在非常规介质中，特别是有机溶剂介质（OSM）中的机械作用和生物催化。虽然候选人进行的研究工作解决了OSM中脂氧合酶/氢过氧化物裂解酶系统的固定化和生物催化，但这些酶的稳定性以及它们在OSM中的作用机理的阐明仍然是有待克服的主要挑战之一。获得的知识演讲使我们在研究中走向新的视野，特别是使用纳米技术方法进行酶固定化以及建模和ioinformatics设置实验设计和调查酶-底物相互作用。