NSERC/MDS Analytical Technologies Industrial Research Chair in Analytical Mass Spectrometry

该提案是为NSERC / MDS分析技术工业研究主席在分析质谱的第三个任期的更新。该IRC与工业合作伙伴MDS Analytical Technologies（AT，前身为SCIEX）合作，在开发质谱（MS）和应用程序的新型硬件方面进行研究。该更新提案包括正在进行的合作项目和与MDS AT开发的新项目。按照目前的做法，许多这些项目将在约克大学的主席实验室和他的合作者在MDS AT的实验室进行。Siu的研究生，博士后研究员和MDS AT工作人员将能够在两个地点进行研究，这得益于两个合作机构之间的短暂物理分离（乘车15分钟）。主席的研究计划的一个重要部分将致力于了解和开发新的离子解离方法和仪器。其中包括红外多光子解离，由主席团队首次在混合四极杆/飞行时间（QqTOF）质谱仪（MDS AT QSTAR的原型）中实施;和紫外解离，这是一个新项目，其初步数据显示相当有前途。这项研究的结果将导致更好地了解这些解离方法的潜力，用于实施的QqTOF和其他类型的MS/MS仪器，由MDS AT制造，并可能导致商业产品。同样重要的是致力于更好地定量分析肽和小分子药物的项目。这些项目包括调查多反应监测、使用微分迁移率分析仪和增强多电荷扫描以提高快速分离的选择性，以及从增强量化的角度简化离子分布的离子停放。此外，将致力于在第二个主席任期内开发的新型仪器中应用离子迁移率光谱法，该仪器将离子迁移率和三重四极杆MS集成在一起，用于补充MS的药物代谢物表征。

This proposal is for renewal of a third term of the NSERC / MDS Analytical Technologies Industrial Research Chair in Analytical Mass Spectrometry. This IRC conducts research in developing novel hardware in mass spectrometry (MS) and applications in collaboration with MDS Analytical Technologies (AT, formerly SCIEX), the Industrial Partner. This proposal for renewal comprises ongoing collaborative projects and new projects developed with MDS AT. As in current practice, many of these projects will be carried out in both the Chair's laboratories in York University and his collaborators' laboratories in MDS AT. Siu's graduate students, postdoctoral fellows, and MDS AT staff will be able to conduct research in both locations, facilitated by the short physical separation (-15 minutes by car) between the two collaborating institutions. A significant portion of the Chair's research program will be devoted to understanding and developing of new ion dissociation methods and instrumentation. These include infrared multiphoton dissociation, implemented for the first time in a hybrid quadrupole / time-of-flight (QqTOF) mass spectrometer (a prototype of the MDS AT QSTAR) by the Chair's team; and ultraviolet dissociation, a new project whose preliminary data show considerable promise. Results of this research will lead to a better understanding of the potential of these dissociation methods for implementation on the QqTOF and other types of MS/MS instrumentation that are manufactured by MDS AT, and may lead to commercial products. Equally significant are projects devoted to better quantitative analyses of peptide and small-molecule drugs. These projects include investigations into multiple-reaction monitoring, the use of a differential mobility analyzer and the enhanced multiply charge scan for improved selectivity in fast separations, and ion parking for simplifying ion distributions from the perspective of enhancing quantification. In addition, efforts will be devoted to apply ion mobility spectrometry in a novel instrumentation developed during the second Chair term that integrates ion mobility and triple quadrupole MS for drug metabolite characterization that complements MS.