Control of transcription by the cardiac homeodomain protein Nkx2-5

心脏同源结构域蛋白 Nkx2-5 对转录的控制

• 批准号: ARC : DP0212000
• 负责人: Richard Harvey
• 金额: $ 23.1万
• 依托单位: Victor Chang Cardiac Research Institute
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/control-transcription-cardiac-nkx2-5/72873
• 关键词: Control; transcription; cardiac; homeodomain; protein

Control of transcription by the cardiac homeodomain protein Nkx2-5. The transcriptional regulatory protein Nkx2-5, a member of the homeodomain superfamily, is essential for heart development and mutations in the human gene cause congenital heart disease. We seek to define the molecular mechanisms that regulate the transcriptional activity of Nkx2-5. We have previously identified a transcriptional activation domain in the C-terminal region that is bipartite in nature and conserved among Nkx2-5 proteins from diverse species. We will characterise the consequences of mutations in this domain in mouse models and search for interacting proteins. Results will advance our understanding of gene regulation in the context of heart disease.

心脏同源结构域蛋白Nkx 2 -5的转录控制。转录调节蛋白Nkx 2 -5是同源结构域超家族的成员，对心脏发育至关重要，人类基因突变导致先天性心脏病。我们试图确定的分子机制，调节Nkx 2 -5的转录活性。我们以前已经确定了一个转录激活结构域的C-末端区域是二分的性质和保守的Nkx 2 -5蛋白从不同的物种。我们将在小鼠模型中研究该结构域突变的后果，并寻找相互作用的蛋白质。研究结果将促进我们对心脏病背景下基因调控的理解。