Defining the pathways for the import of proteins into mitochondria

定义蛋白质导入线粒体的途径

• 批准号: ARC : DP0208309
• 负责人: Michael Ryan
• 金额: $ 18万
• 依托单位: La Trobe University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/defining-pathways-import-proteins-mitochondria/74896
• 关键词: Defining; pathways; import; proteins; into

Defining the pathways for the import of proteins into mitochondria. This application deals with the targeting of preproteins from the cytosol to the mitochondria in mammalian cells. The aims are to identify and characterise mitochondrial targeting signals within preproteins lacking typical N-terminal signal sequences; to characterise the roles of the cytosolic molecular chaperones such as Hsp90 and Hsp70 in assisting the movement of preproteins to the mitochondrial surface; to determine the interactions of both preproteins and cytosolic factors with outer membrane components and; to analyse the roles of individual TOM receptors in import. The results obtained will facilitate our understanding of general protein import processes and those specific for mammals.

确定蛋白质进入线粒体的途径。这个应用程序处理从细胞质到线粒体的前蛋白在哺乳动物细胞中的靶向。目的是在缺乏典型n端信号序列的前蛋白中识别和表征线粒体靶向信号；描述胞质分子伴侣如Hsp90和Hsp70在协助前蛋白向线粒体表面移动中的作用；确定前蛋白和细胞质因子与外膜组分的相互作用；分析TOM受体在进口中的作用。所获得的结果将有助于我们理解一般的蛋白质输入过程和哺乳动物特有的蛋白质输入过程。