Investigating immunomodulating mechanisms of probiotics

研究益生菌的免疫调节机制

• 批准号: 312482-2008
• 负责人: Kim, SungOuk
• 金额: $ 2.09万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=457444
• 关键词: Investigating; immunomodulating; mechanisms; probiotics

Probiotics are 'living micro-organisms that when administered in adequate amounts confer a health benefit to the host'. An increasing number of studies provide scientific evidence for the efficacy and efficiency of probiotics in alleviating several inflammatory diseases. However, the specific mechanisms by which probiotic bacteria contribute to health, and, especially, their anti-inflammatory properties remain poorly understood. This research program is to investigate the signaling and molecular immunomodulatory mechanisms of probiotics. We have shown that probiotic strains of Lactobacillus rhamnosus are potent inducers of G-CSF production, which suppresses production of the pro-inflammatory cytokine tumor necrosis factor (TNF) in activated macrophages. We hypothesize that L. rhamnosus preferentially induces G-CSF in monocytes/macrophages, which is a key anti-inflammatory cytokine mediating immunomodulatory effects on macrophages and dendritic cells. To address the hypothesis, we propose the following four specific research objectives: 1) Investigating the signaling mechanism of G-CSF production in L. rhamnosus-activated macrophages, 2) Investigating the effect of G-CSF on macrophages, 3) Investigating the mechanism of G-CSF in regulating macrophage activation and 4) Investigating the role of G-CSF on dendritic cells.

益生菌是“活的微生物，当给予足够的量时，会给宿主带来健康益处”。越来越多的研究为益生菌在减轻几种炎症性疾病方面的功效和效率提供了科学证据。然而，益生菌促进健康的具体机制，特别是其抗炎特性仍然知之甚少。本课题旨在探讨益生菌的信号和分子免疫调节机制。我们已经证明，鼠李糖乳杆菌益生菌菌株是G-CSF产生的有效诱导剂，G-CSF抑制活化巨噬细胞中促炎细胞因子肿瘤坏死因子（TNF）的产生。我们假设鼠李糖在单核/巨噬细胞中优先诱导G-CSF，而G-CSF是介导巨噬细胞和树突状细胞免疫调节的关键抗炎细胞因子。为了验证这一假设，我们提出了以下四个具体的研究目标：1)研究鼠李糖活化巨噬细胞中G-CSF产生的信号机制，2)研究G-CSF对巨噬细胞的作用，3)研究G-CSF调节巨噬细胞活化的机制，4)研究G-CSF对树突状细胞的作用。