Ozone free box for microarray based functional genomics studies

用于基于微阵列的功能基因组学研究的无臭氧盒

• 批准号: 406629-2011
• 负责人: Dixon, Walter
• 金额: $ 1.62万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472524
• 关键词: Ozone; free; box; microarray; based

High throughput functional genomics and proteomics studies are the major focus of the research conducted in a multi-user Agricultural Genomics and Proteomics Unit (AGP Unit) in the Department of Agricultural, Food and Nutritional Science. Various researchers from The Alberta Ingenuity Centre for Livestock Genomics, The Dairy Nutritional Research Group, The Swine Reproduction-Development Program/Embryogene Network, Plant Biotechnology and plant pathology group, Human Nutrition program, Immunology, Food microbiology, Functional Foods and Nutraceuticals group, and scientists at Agriculture and Agri-Food Canada, are currently supporting the research programs of over 100 users. All these groups are applying innovative efforts to improve production and reproductive performance of livestock, developing animal products with improved nutritional qualities, understanding molecular mechanisms of host-microbial interactions and creating crops with improved disease resistance and value added characteristics. DNA and protein microarrays are widely used by most of the researchers at the AGP Unit. BioTray®'s Ozone Free BoxTM is a powerful solution which totally destroys ozone in order to have an ozone free environment during microarray sample preparation, hybridization, washes, drying and scanning stages. With this ozone free space, highly reproducible data from microarray with minimal technical variation will be generated and this is particularly important to accurately quantify the transcripts in a given cell or tissue under different treatments or physiological conditions. This should also significantly improve image quality of the scanned microarray slides with increased reproducibility and data quality after scanning. The availability of the microarray hybridization station is crucial and required for a variety of applications by research projects of 9 major users as well as additional minor users within this large core facility. This will further facilitate the training of students, technicians and postdocs in current genomics technologies. The research supported by this infrastructure will play an important role towards the future sustainability and profitability of Canadian agriculture industries.

高通量功能基因组学和蛋白质组学研究是农业，食品和营养科学系多用户农业基因组学和蛋白质组学单位（AGP单位）进行的研究的主要重点。来自阿尔伯塔家畜基因组学研究中心、乳制品营养研究组、猪繁殖发展计划/胚胎基因网络、植物生物技术和植物病理学组、人类营养计划、免疫学、食品微生物学、功能食品和营养品组的各种研究人员以及加拿大农业和农业食品的科学家目前正在支持100多个用户的研究计划。所有这些团体都在进行创新努力，以提高牲畜的生产和繁殖性能，开发具有更好营养质量的动物产品，了解宿主-微生物相互作用的分子机制，并创造具有更好抗病性和增值特性的作物。DNA和蛋白质微阵列被AGP单位的大多数研究人员广泛使用。BioTray®的Ozone Free Box TM是一种功能强大的解决方案，它完全破坏臭氧，以便在微阵列样品制备，杂交，洗涤，干燥和扫描阶段拥有无臭氧的环境。利用这种无臭氧空间，将以最小的技术变化产生来自微阵列的高度可再现的数据，这对于在不同治疗或生理条件下准确定量给定细胞或组织中的转录物特别重要。这也将显著改善扫描的微阵列载玻片的图像质量，增加扫描后的再现性和数据质量。微阵列杂交站的可用性至关重要，并且对于这个大型核心设施内的9个主要用户以及其他次要用户的研究项目的各种应用来说都是必需的。这将进一步促进对学生、技术人员和博士后进行当前基因组学技术方面的培训。该基础设施支持的研究将对加拿大农业未来的可持续性和盈利能力发挥重要作用。