Real-time PCR thermal cycler for environmental response transcriptomics and epigenetics

用于环境响应转录组学和表观遗传学的实时 PCR 热循环仪

• 批准号: 407563-2011
• 负责人: Campbell, Malcolm
• 金额: $ 3.08万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472659
• 关键词: Real; time; PCR; thermal; cycler

The organization of DNA into chromatin presents the cell with the opportunity to use powerful regulatory mechanisms broadly defined as epigenetics. Increasing evidence demonstrates that epigenetic mechanisms are linked to gene activation, gene silencing and chromosomal instability. The research program entails examination of several aspects of epigenetic regulation, from DNA methylation at cis-regulatory regions, to chromatin modifications, to non-coding RNA. State-of-the-art technology to examine these changes involves using quantitative real-time PCR as a principal tool to gain insight into environmental effects on the regulation of gene expression.

DNA进入染色质的组织为细胞提供了使用广泛定义为表观遗传学的强大调控机制的机会。越来越多的证据表明，表观遗传机制与基因激活、基因沉默和染色体不稳定有关。该研究计划需要检查表观遗传调控的几个方面，从顺式调节区的DNA甲基化，到染色质修饰，再到非编码RNA。检查这些变化的最先进技术包括使用定量实时聚合酶链式反应作为主要工具，以洞察环境对基因表达调控的影响。