Real-time PCR thermal cycler for environmental response transcriptomics and epigenetics
Real-time PCR thermal cycler for environmental response transcriptomics and epigenetics
批准号:
407563-2011
负责人:
Campbell, Malcolm
金额:
$3.08万
依托单位:
依托单位国家:
加拿大
项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
财政年份:
2010
资助国家:
加拿大
项目状态:
已结题
起止时间:
2010-01-01 至 2011-12-31
中文摘要
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英文摘要
The organization of DNA into chromatin presents the cell with the opportunity to use powerful regulatory mechanisms broadly defined as epigenetics. Increasing evidence demonstrates that epigenetic mechanisms are linked to gene activation, gene silencing and chromosomal instability. The research program entails examination of several aspects of epigenetic regulation, from DNA methylation at cis-regulatory regions, to chromatin modifications, to non-coding RNA. State-of-the-art technology to examine these changes involves using quantitative real-time PCR as a principal tool to gain insight into environmental effects on the regulation of gene expression.
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