Chromatin remodeling and nuclear reprogramming in domestic animal clones and embryonic stem cells

家畜克隆和胚胎干细胞的染色质重塑和核重编程

• 批准号: 250191-2007
• 负责人: Betts, Dean
• 金额: $ 2.19万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=479512
• 关键词: Chromatin; remodeling; nuclear; reprogramming; domestic

The production of viable embryos, embryonic stem cell lines, and live animals using cloning technologies has confirmed that sufficient reversal of differentiated fetal and adult genomes can be achieved. However, high embryonic, fetal and neonatal deaths with abnormalities affecting the placenta, the respiratory and circulatory systems and growth of the fetus and neonate clone greatly lower the live birth rate to ~1-5% indicating that this reprogramming process is inadequate most of the time.

利用克隆技术生产有活力的胚胎、胚胎干细胞系和活体动物已经证实，可以实现分化的胎儿和成体基因组的充分逆转。 然而，胚胎、胎儿和新生儿死亡率高，异常影响胎盘、呼吸和循环系统以及胎儿和新生儿克隆的生长，大大降低了活产率至约1-5%，表明这种重编程过程在大多数情况下是不充分的。