Dynamics of myosin filaments in single smooth muscle cells

单个平滑肌细胞中肌球蛋白丝的动力学

• 批准号: 355639-2008
• 负责人: Leguillette, Renaud
• 金额: $ 2.17万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=482796
• 关键词: Dynamics; myosin; filaments; single; smooth

Smooth muscle (SM) was described for the first time in the early 1900s, but contrary to striated muscle, the structure and mechanics of SM contractile filaments is still poorly understood. The unique functional properties of SM are incompatible with a fixed array of myosin filaments. Little is known on the assembly of myosin isoforms SM1 and SM2 in vivo. Models have proposed that myosin filaments are labile and can reorganize to generate maximal tension in vivo, but this have never been directly shown. The few studies that did not use stationary images reported changes in the birefringence and X-ray diffraction patterns in SM during contraction. These techniques are, however, not specific for myofilaments. Therefore, it seems that the best way to examine the dynamics of myofilaments is to directly observe them in real-time.

平滑肌（SM）在20世纪初首次被描述，但与横纹肌相反，SM收缩丝的结构和力学仍然知之甚少。SM的独特功能特性与肌球蛋白丝的固定阵列不相容。关于肌球蛋白亚型SM 1和SM 2在体内的组装知之甚少。模型已经提出肌球蛋白丝是不稳定的，并且可以重组以在体内产生最大张力，但这从未被直接证明。少数研究没有使用静态图像报告收缩过程中SM的双折射和X射线衍射图案的变化。然而，这些技术并不特异于肌丝。因此，研究肌丝动力学的最佳方法似乎是直接实时观察它们。