Influence of ABCA1 on transbilayer distribution of cholesterol in mammalian cells

ABCA1 对哺乳动物细胞胆固醇跨双层分布的影响

• 批准号: 402010-2011
• 负责人: Zha, Xiaohui
• 金额: $ 2.19万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=487905
• 关键词: Influence; ABCA1; transbilayer; distribution; cholesterol

Cholesterol is a major component of mammalian cells, particularly in the plasma membrane (PM). In the PM, cholesterol is the basic building block and also essential in forming heterogeneous microdomains such as lipid raft, an entity important in signaling and membrane trafficking (1). Proposed NSERC program is to develop novel biophysical and microscopy/imaging techniques to understand how cholesterol is organized in the PM. The immediate objective for next 5-years is to determine cholesterol transbilayer distribution in the PM. As such, proposed program will study a fundamental issue in mammalian cells and, at the same time, compliment a non-overlapping research program in my laboratory funded by HSFO, which focuses on protein-mediated cholesterol export from mammalian cells. The PM is the main platform for this cholesterol export. Advance in understanding of PM cholesterol organization will facilitate our study on cholesterol export.

胆固醇是哺乳动物细胞的主要成分，特别是在质膜（PM）中。在PM中，胆固醇是基本的结构单元，也是形成异质微区（如脂筏）所必需的，脂筏是信号传导和膜运输中的重要实体（1）。拟议的NSERC计划是开发新的生物物理和显微镜/成像技术，以了解胆固醇是如何组织在PM。未来5年的直接目标是确定PM中的胆固醇跨双层分布。因此，拟议的计划将研究哺乳动物细胞中的一个基本问题，同时，补充由HSFO资助的我的实验室中的一个非重叠研究计划，该计划侧重于蛋白质介导的哺乳动物细胞胆固醇输出。首相是这种胆固醇出口的主要平台。对PM胆固醇组织的进一步了解将有助于我们对胆固醇输出的研究。