Components to support a Spinning Disk Confocal Microscope

支持转盘共焦显微镜的组件

• 批准号: 423271-2012
• 负责人: Coe, Imogen
• 金额: $ 10.25万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495911
• 关键词: Components; support; Spinning; Disk; Confocal

A number of researchers at York University study cells using techniques such as microscopy. We are particularly interested in how things work inside cells and traditionally have studied different parts of cells and different activities in cells using microscopes that can only look at cells that have been "fixed". Fixed cells are cells that have been suddenly stopped in their tracks (fixed) so that the structure and layout of the cells are maintained but they are dead and nothing is functioning in them. There are now more advanced microscopes that will allow us to look at living cells and to record activities of cellular functions in real time. This is obviously a powerful approach to study living cells and see how various parts of the cells are behaving in different conditions and perhaps with different types of treatments. In this application, we are requesting support for some components of a new microscope that we have acquired which will allow a large number of researchers to investigate various aspects of cell biology, using live cell imaging on a spinning disk confocal microscope.

约克大学的一些研究人员使用显微镜等技术研究细胞。 我们对细胞内部的工作方式特别感兴趣，传统上，我们使用显微镜研究细胞的不同部分和细胞的不同活动，显微镜只能观察已经“固定”的细胞。 固定细胞是突然停止在其轨道上的细胞（固定），因此细胞的结构和布局保持不变，但它们是死的，没有任何功能。 现在有更先进的显微镜，使我们能够观察活细胞，并在真实的时间内记录细胞功能的活动。 这显然是一种研究活细胞的强大方法，可以观察细胞的各个部分在不同条件下以及不同类型的治疗下的行为。 在这个应用程序中，我们正在请求支持我们已经获得的新显微镜的一些组件，这将允许大量的研究人员调查细胞生物学的各个方面，使用活细胞成像旋转盘共聚焦显微镜。