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Analysis of dynamics and protein turnover in integrin-mediated adhesion in drosphilia muscles.

果蝇肌肉整合素介导的粘附的动力学和蛋白质周转分析。

基本信息

批准号：
356502-2008
负责人：
Tanentzapf, Guy
金额：
$ 2.7万
依托单位：
University of British Columbia
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2012
资助国家：
加拿大
起止时间：
2012-01-01 至 2013-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498147
关键词：
Analysis dynamics protein turnover integrin

项目摘要

Cells in multicellular organisms are arranged in a remarkably diverse array of structures. My main scientific interest is in determining how cells come together to form complex structures and then, once formed, how such structures are maintained. I am interested in the basic cellular mechanisms that determine how cells interact with each other and with their environment. In particular I am interested in how cell adhesion is utilized during animal development. I use one type of adhesion, cell adhesion to the extracellular matrix, as a model to address fundamental question about the role of adhesion in development. Adhesion receptors of the integrin family are the core components of an adhesion complex that is important for the attachment of cells to the extracellular matrix. The disassembly and assembly of integrin adhesions is carefully regulated but it is currently not known in what ways the precise regulation of the turnover of integrin contributes to animal development. I have shown that it is possible to visualize the assembly and dynamics of integrin adhesion complexes using live imaging of tagged integrin associated proteins in embryonic muscles. I have shown that the dynamics of integrin adhesion vary during development. Many of the proteins associated with integrin adhesion are GFP-tagged and modifications in integrin-associated proteins that alter the dynamics of integrin adhesions in cell culture are now available in flies. I will carry out a systematic survey of turnover of integrin adhesion dynamics within the fly.

在多细胞生物中，细胞以非常多样化的结构排列。我的主要科学兴趣是确定细胞如何聚集在一起形成复杂的结构，然后，一旦形成，如何维持这些结构。我对基本的细胞机制感兴趣，这些机制决定了细胞如何相互作用，以及细胞如何与环境相互作用。我特别感兴趣的是在动物发育过程中如何利用细胞粘附。我用一种类型的粘附，细胞粘附到细胞外基质，作为一个模型来解决关于粘附在发育中的作用的基本问题。整合素家族的粘附受体是粘附复合物的核心成分，对于细胞粘附到细胞外基质非常重要。整合素粘附体的拆卸和组装受到精心调节，但目前尚不清楚整合素周转的精确调节以何种方式促进动物发育。我已经证明，利用胚胎肌肉中标记的整合素相关蛋白的实时成像，可以可视化整合素粘附复合物的组装和动力学。我已经展示了在发育过程中整合素粘附的动态变化。许多与整合素粘附相关的蛋白质是gfp标记的，整合素相关蛋白的修饰改变了细胞培养中整合素粘附的动力学，现在可以在苍蝇中使用。我将对苍蝇体内整合素粘附动力学的周转进行系统的调查。