Which variants of influenza viruses commonly circulate in Ontario swine?

拟议工作的目的是确定哪种类型的流感病毒在安大略省的猪中传播。这将通过对代表性流感病毒分离株进行全序列分析来实现。通过对本研究中流感病毒检测呈阳性的样本的血凝素（HA）流感病毒糖蛋白编码基因片段进行系统发育分析，并通过胚胎鸡蛋的病毒学测试进行评估，从而确定什么是具有代表性的流感病毒分离物。研究样本将通过以下两种抽样机制之一收集：(i)定期访猪群；（ii）调查猪场的流感样疾病。现场样本（即鼻拭子）将由圭尔夫大学人口医学系的现场工作人员或兽群兽医提交给研究实验室。为了收集样本，将使用标准程序。这一信息对于疫苗生产商生产最新的猪流感疫苗非常重要。此外，生产商必须在知情的情况下做出决定，选择与安大略省流行的病毒株最相似的疫苗。本研究的信息也可用于追踪安大略省猪群中优势菌株的长期趋势。

The objective of the proposed work is to determine which types of influenza viruses circulate in Ontario swine. This will be achieved by full sequence analysis of representative influenza virus isolates. What is a representative influenza virus isolate will be determined by doing phylogenetic analysis of the gene segments encoding the hemagglutinin (HA) influenza virus glycoproteins of samples that tested positive for influenza virus in this study, as evaluated by virological test in embryonated chicken eggs. Samples for the study will be collected through one of the two sampling mechanisms: (i) regular herd visits, (ii) investigation of influenza-like disease on swine farms. Field samples (ie, nasal swabs) will be submitted to the research laboratory either by a field crew from the Department of Population Medicine (University of Guelph), or by herd veterinarians. To collect samples, a standard protocol will be used. This information will be important for vaccine manufacturers in order to produce the most updated vaccine for influenza in swine. In addition, it will be important for producers to make informed decisions about the choice of vaccines that would have the strains most similar to strains that circulate in Ontario. The information in this study could also be used for tracing long-term trends of dominant strains in the Ontario swine population.