Which variants of influenza viruses commonly circulate in Ontario swine?

哪些流感病毒变种通常在安大略省猪中传播？

• 批准号: 428602-2011
• 负责人: Poljak, Zvonimir
• 金额: $ 1.46万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=498307
• 关键词: variants; influenza; viruses; commonly; circulate

The objective of the proposed work is to determine which types of influenza viruses circulate in Ontario swine. This will be achieved by full sequence analysis of representative influenza virus isolates. What is a representative influenza virus isolate will be determined by doing phylogenetic analysis of the gene segments encoding the hemagglutinin (HA) influenza virus glycoproteins of samples that tested positive for influenza virus in this study, as evaluated by virological test in embryonated chicken eggs. Samples for the study will be collected through one of the two sampling mechanisms: (i) regular herd visits, (ii) investigation of influenza-like disease on swine farms. Field samples (ie, nasal swabs) will be submitted to the research laboratory either by a field crew from the Department of Population Medicine (University of Guelph), or by herd veterinarians. To collect samples, a standard protocol will be used. This information will be important for vaccine manufacturers in order to produce the most updated vaccine for influenza in swine. In addition, it will be important for producers to make informed decisions about the choice of vaccines that would have the strains most similar to strains that circulate in Ontario. The information in this study could also be used for tracing long-term trends of dominant strains in the Ontario swine population.

拟议工作的目的是确定哪种类型的流感病毒在安大略猪中传播。这将通过对代表性流感病毒分离株进行全序列分析来实现。通过对本研究中流感病毒检测阳性样本的血凝素（HA）流感病毒糖蛋白编码基因片段进行系统发育分析（通过鸡胚中的病毒学检测进行评价），确定代表性流感病毒分离株。将通过以下两种采样机制之一采集研究样本：（i）定期牛群访视，（ii）猪场流感样疾病调查。田间样本（即鼻拭子）将由人口医学系（圭尔夫大学）的现场工作人员或畜群兽医提交给研究实验室。为了收集样本，将使用标准方案。这些信息对于疫苗生产商来说非常重要，以便生产最新的猪流感疫苗。此外，重要的是，生产商应就疫苗的选择做出明智的决定，这些疫苗的菌株与安大略流行的菌株最相似。本研究中的信息也可用于追踪安大略猪群中优势菌株的长期趋势。