Enteric neural regulation of barrier function in the intestine

肠屏障功能的肠神经调节

• 批准号: 424188-2012
• 负责人: MacNaughton, Wallace
• 金额: $ 1.97万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Group
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506241
• 关键词: Enteric; neural; regulation; barrier; function

The single layer of epithelial cells that line the gastrointestinal (GI) tract is an essential barrier between the external environment and the inside of the body. The ability to regulate permeability through this barrier resides at the level of the apical junctional complex. The apical junctional complex consists of the tight and adherence junctions of the epithelium and is one of two barriers to movement through the intestinal epithelium; the other being a secretory barrier. The secretory barrier includes antimicrobial peptides, mucus and fluid. Considerable work has revealed that secretion is regulated by neural mechanisms that integrate barrier function with digestive processes in the gut. By comparison, far less is known about the neural control of the apical junctional complex on the intestine. We aim to address this significant gap in our knowledge by testing the general hypothesis that the innervation of the intestine regulates epithelial barrier function by altering the expression and localization of tight junction proteins.

胃肠道(GI)内的单层上皮细胞是外部环境和身体内部之间的重要屏障。通过这种屏障调节通透性的能力存在于顶端连接复合体的水平。顶端连接复合体由上皮的紧密连接和粘连连接组成，是通过肠上皮移动的两个障碍之一；另一个是分泌障碍。分泌屏障包括抗菌肽、粘液和液体。大量的研究表明，分泌是由神经机制调节的，这种机制将肠道中的屏障功能与消化过程结合起来。相比之下，人们对肠道顶端连接复合体的神经控制知之甚少。我们的目标是通过测试肠神经支配通过改变紧密连接蛋白的表达和定位来调节上皮屏障功能的普遍假设来解决我们知识中的这一显着差距。