Protein Interactions and Solvation in Binary Solvents

二元溶剂中的蛋白质相互作用和溶剂化

• 批准号: 203816-2012
• 负责人: Chalikian, Tigran
• 金额: $ 3.28万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=514139
• 关键词: Protein; Interactions; Solvation; Binary; Solvents

We propose to continue our studies of the effect of solvation on the conformational preferences and interactions of proteins. During the requested budget period, we propose to carry out volumetric and spectroscopic experiments as well as molecular dynamics (MD) simulations which are designed to achieve the following specific aims: comparative and syste¬matic investigation of solvation of the 20 naturally existing amino acid side chains in N-acetyl amino acid amides in water and binary mixtures of water and water-soluble denaturing and stabilizing cosolvents; thermodynamic characterization of the association of various cosolvents with individual protein groups; volumetric characterization of solvation of native and unfolded proteins in denaturing and stabilizing cosolvents and exploration of the ad¬ditivity of protein sol¬vation; and comparative characterization of protein folding and binding reactions in water and denaturing and stabilizing cosolvents. The domineering influence of solvent on chemical equi¬libria involving proteins is unanimously acknowledged. In fact, any protein reaction in an aqu¬eous solution can be considered as replacement of solute-solvent with solute-solute interac¬tions. With this notion, it is clear that the thermodynamics of solute-solvent and solute-cosolvent interactions is an important integral part of the reaction thermodynamics. Our proposed volu¬met¬ric studies on low molecular weight model compounds amplified by the statistical thermo¬dynamic interpretation algorithm and MD simulations will enable us to quantify the differential affinity of protein functional groups for water and stabilizing and denaturing osmolytes. Exten¬ding these studies to proteins will permit us to investigate nonadditive solvation effects in the folded and unfolded conformations. Finally, characterization of protein folding and binding reactions in binary solvents will test our basic conclusions on real biological systems.

我们建议继续研究溶剂化对蛋白质构象偏好和相互作用的影响。在要求的预算期内，我们拟开展体积和光谱实验以及分子动力学（MD）模拟，旨在实现以下具体目标：比较和系统地研究20种天然存在的氨基酸侧链在n -乙酰氨基酸酰胺中在水和水与水溶性变性和稳定共溶剂的二元混合物中的溶剂化；不同助溶剂与单个蛋白质基团缔合的热力学表征天然和未折叠蛋白质在变性和稳定共溶剂中溶剂化的体积表征及蛋白质溶剂化的附加性探索并比较表征了蛋白质在水和变性和稳定共溶剂中的折叠和结合反应。溶剂对涉及蛋白质的化学平衡的决定性影响是公认的。事实上，水溶液中的任何蛋白质反应都可以认为是溶质-溶质相互作用取代了溶质-溶质相互作用。有了这个概念，很明显，溶质-溶剂和溶质-助溶剂相互作用的热力学是反应热力学的重要组成部分。我们提出的通过统计热力学解释算法和MD模拟放大的低分子量模型化合物的体积研究将使我们能够量化蛋白质官能团对水和稳定和变性渗透物的差异亲和力。将这些研究扩展到蛋白质将使我们能够研究折叠和未折叠构象中的非加性溶剂化效应。最后，在二元溶剂中蛋白质折叠和结合反应的表征将在实际生物系统中验证我们的基本结论。