Nutritional regulation of rumen epithelium

瘤胃上皮的营养调节

• 批准号: 2873-2010
• 负责人: McBride, Brian
• 金额: $ 4.44万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=520519
• 关键词: Nutritional; regulation; rumen; epithelium

Commercial dairy production systems rely on energy dense diets with elevated levels of rapidly fermentable carbohydrates to improve production efficiency. However, consumption of high grain/low-fibre diets by ruminants commonly leads to an accumulation of volatile fatty acids (VFAs) and lactate within the rumen causing ruminal acidosis. The impacts of acidosis include reduced feed intake, rumen epithelium damage and, subsequently, can lead to liver damage, laminitis and death. This program of study will characterize an acidosis-induction model and the impacts of the disease on rumen epithelial cell turnover and expression of genes regulating protein turnover. This comprehensive biological model will allow subsequent development of nutritional strategies to enhance rumen epithelium adaptation and recovery to ruminal acidosis. The central hypothesis is; in lactating cows suffering from subacute ruminal acidosis the reduction of ruminal lactate along with therapeutic treatment with leucine will reduce protein degradation pathways and increase cell proliferation of the rumen epithelium. This program of study will lead to improvements in the digestive function, productivity and well-being of lactating dairy cows.

商业乳制品生产系统依靠能量密集的日粮和高水平的快速发酵碳水化合物来提高生产效率。然而，反刍动物食用高谷物/低纤维饲料通常会导致瘤胃内挥发性脂肪酸(VFAS)和乳酸的积累，从而导致瘤胃酸中毒。酸中毒的影响包括采食量减少，瘤胃上皮受损，随后可能导致肝脏损伤、板层炎和死亡。这项研究计划将描述酸中毒诱导模型以及疾病对瘤胃上皮细胞周转和调节蛋白质周转的基因表达的影响。这一全面的生物学模型将允许后续开发营养策略，以增强瘤胃上皮对瘤胃酸中毒的适应和恢复。中心假设是：在患有亚急性瘤胃酸中毒的哺乳奶牛中，瘤胃乳酸的减少以及亮氨酸的治疗将减少蛋白质降解途径，并促进瘤胃上皮细胞的增殖。这项研究计划将有助于改善哺乳奶牛的消化功能、生产力和福祉。