The cellular biology of turnip mosaic virus

萝卜花叶病毒的细胞生物学

• 批准号: 3452-2008
• 负责人: Laliberté, JeanFrançois
• 金额: $ 3.72万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=520584
• 关键词: cellular; biology; turnip; mosaic; virus

For many positive-strand RNA [(+) RNA] viruses, RNA replication occurs within cytoplasmic membranous vesicles. Over the past years, my research team has shown that the protein linked to the genome (VPg) of Turnip mosaic virus (TuMV) interacts with several virus and host proteins, notably translation factors. We have found that many of these interactions are taking place in replication vesicles induced by 6K-VPg-Pro, a precursor form of VPg. In this research proposal, I wish to extend our comprehension of the vesicles harbouring the virus replication complex. More specifically, I wish to elucidate their content and architecture and study their genesis and movement within the cell.

对于许多正链RNA (+) RNA)病毒，RNA复制发生在细胞质膜囊内。在过去的几年里，我的研究小组已经证明，芜菁花叶病毒（TuMV）基因组连接蛋白（VPg）与几种病毒和宿主蛋白相互作用，特别是翻译因子。我们发现许多这些相互作用发生在由6K-VPg-Pro （VPg的前体形式）诱导的复制囊泡中。在这个研究计划中，我希望扩展我们对窝藏病毒复制复合体的囊泡的理解。更具体地说，我希望阐明它们的内容和结构，并研究它们在细胞内的起源和运动。