Studies on prolactin in galliformes

鸡形目催乳素的研究

• 批准号: 43832-2010
• 负责人: Zadworny, David
• 金额: $ 1.97万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=521685
• 关键词: Studies; prolactin; galliformes

A problem of considerable economic importance to the poultry producer is the occurrence of incubation behavior ("broodiness") in breeder hens. This behavior is characterized by increased nesting frequency, incubation of eggs, involution of the ovary, anorexia, adipsea and large changes in intermediary metabolism. As a result of the expression of this trait, substantial costs are incurred due to both the loss of egg production and to the more intensive labour/management procedures that are required for prevention. The pituitary hormone, prolactin (PRL) is thought to play a key role in broodiness since blood levels increase 6 to 10 fold at the onset and are maintained at high levels throughout the expression of the behavior. Among vertebrate species, greater than 300 biological actions of PRL have been described but how PRL is involved in the physio-behavioural repertoire associated incubation is unknown. We have recently noted that up to 8 different post-translationally modified forms of PRL (glycosylation, phosphorylation) are secreted by the pituitary and the ratio of these forms varies greatly during the life cycle of chickens and turkeys. Notably, the modified forms are dominant (>75 %) during incubation behavior and during the late stages of embryogenesis. This is an unexpected result since mammalian PRLs generally have reduced binding affinity to the receptor and are more rapidly cleared from the blood. The avian PRL receptor may be activated in specific tissues differently at specific stages of the life cycle by these altered PRLs. In order to study, the effects of PRL we plan 1) to knock down expression of PRL using RNA interference 2) to purify the various isoforms and test receptor activation 3) to continue genetic analysis of this and other candidate genes which can be used for marker assisted selection to reduce broodiness in breeder hens.

对家禽生产者来说，一个相当重要的经济问题是种鸡的孵化行为(“就巢”)的发生。这种行为的特征是筑巢频率增加，卵孵化，卵巢退化，厌食，脂肪减少，中间代谢发生重大变化。由于这一特点的表现，由于鸡蛋生产的损失和预防所需的更密集的劳动力/管理程序，造成了巨大的成本。垂体腺激素催乳素(PRL)被认为在就巢中起关键作用，因为血液水平在一开始就增加了6到10倍，并在整个行为表达过程中保持在高水平。在脊椎动物物种中，已描述了PRL的300多种生物学作用，但PRL如何参与与生理行为相关的孵化尚不清楚。我们最近注意到，垂体分泌多达8种不同的翻译后修饰形式的PRL(糖基化、磷酸化)，这些形式的比例在鸡和火鸡的生命周期中变化很大。值得注意的是，在孵化行为和胚胎发育的后期阶段，修饰形式占主导地位(&gt；75%)。这是一个意想不到的结果，因为哺乳动物的PRL通常会降低与受体的结合亲和力，并更快地从血液中清除。在生命周期的特定阶段，这些改变的PRL可能会在特定的组织中以不同的方式激活PRL受体。为了研究PRL的作用，我们计划1)利用RNA干扰抑制PRL的表达；2)纯化各种异构体并测试受体的激活；3)继续对该基因和其他候选基因进行遗传分析，这些基因可以用于标记辅助选择，以减少种鸡的就巢。