Determining the kinetics and mechanism of mRNA export

确定 mRNA 输出的动力学和机制

• 批准号: 386362-2010
• 负责人: Zenklusen, Daniel
• 金额: $ 2.77万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=528843
• 关键词: Determining; kinetics; mechanism; mRNA; export

In eukaryotic cells, the sites of storage of genetic information and of protein synthesis are separated by a double bilipidic membrane impermeable to large molecules. To reach the site of protein synthesis, the messenger RNA (mRNA) has to pass this barrier through the nuclear pore complex. The export of mRNAs however is not an isolated process, it is functionally linked to different upstream and downstream processes, in particular the localization of the gene within the nucleus, transcription and mRNA processing. The long-term objective of our research program is to understand how the interplay between different components of the gene expression pathway result in the efficient and selective export of mRNAs from the nucleus to the cytoplasm. We will therefore link cell biological and biophysical approaches in order to study the process of mRNA export in the closest form possible, the single mRNA expressed from a single gene within a single cell, in real time. Only using such novel technologies, allowing directly observing molecular events within their natural environment, it will be possible to fully understand biological processes occurring in cells.

在真核细胞中，储存遗传信息和蛋白质合成的位点被一层大分子不能透过的双层胆脂膜隔开。为了到达蛋白质合成的位点，信使RNA（mRNA）必须通过核孔复合体穿过这一屏障。然而，mRNA的输出不是一个孤立的过程，它在功能上与不同的上游和下游过程相关，特别是基因在细胞核内的定位、转录和mRNA加工。我们的研究计划的长期目标是了解基因表达途径的不同组分之间的相互作用如何导致mRNA从细胞核到细胞质的有效和选择性出口。因此，我们将结合细胞生物学和生物物理学的方法，以研究mRNA输出的过程中，在最接近的形式可能，从一个单一的基因在一个细胞内表达的单个mRNA，在真实的时间。只有使用这种新技术，允许直接观察自然环境中的分子事件，才有可能完全理解细胞中发生的生物过程。