课题基金 / 基金详情

UV and visible light, fluorescence and luminesence Microtitre Plate Spectrometer

UV and visible light, fluorescence and luminesence Microtitre Plate Spectrometer
紫外和可见光、荧光和发光微量滴定板光谱仪
批准号:
458737-2014
负责人:
Finan, Turlough
金额:
$3.91万
依托单位:
依托单位国家:
加拿大
项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
财政年份:
2013
资助国家:
加拿大
项目状态:
已结题
起止时间:
2013-01-01 至 2014-12-31

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中文摘要
翻译
我们正在申请一种高通量光谱仪平板阅读器,用于检测96和384孔板格式的紫外光、可见光、荧光和发光。这是迫切需要的,因为直到最近还在使用的平板光谱仪不再工作,也不能修理--它必须被更换。这一仪器对于在申请人的实验室中定期进行的定量分析是必不可少的。这些分析包括代谢物浓度、基因表达、酶分析、配体结合分析和细胞生长的测量。检测既可以在活的生物材料上进行,也可以在纯化或部分纯化的蛋白质制剂上进行,偶尔也可以在其他生物材料上进行。培训具有紫外光、可见光、荧光和发光检测能力的光谱仪将提供基本和基本技能,增强我们本科生和研究生的技术专长和经验。
英文摘要
We are applying for a high throughput Spectrometer plate reader for detection of UV light, visible light, fluorescence and luminescence in 96 and 384-well plate-format. This is urgently required as the plate spectrometer that was used until very recently no longer works and cannot be repaired - it must be replaced. This instrument is essential for quantitative assays that are performed in the laboratories of the applicants on a very regular basis. These assays include measurements of metabolite concentrations, gene expression, enzyme assays, ligand binding assays and cell growth. The assays are performed on both live biological materials and purified or partially purified protein preparations, and occasionally on other biological materials. Training on a spectrometer with UV light, visible light, fluorescence and luminescence detection capabilities will provide fundamental and basic skills that enhance the technical expertise and experience of our undergraduate and graduate students.
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