Development of novel assays for the study of peptide-protein interactions using surface plasmon resonance biosensors

该项目旨在解决Nicoya Lifesciences基于表面等离子体共振的相互作用分析系统开发新型肽-蛋白质相互作用测定方案的具体问题。Nicoya有兴趣将其系统的应用扩展到研究市场的生物分子相互作用的热力学和动力学分析，Dieckmann实验室在使用基于表面的方法研究特定蛋白质-肽相互作用方面经验丰富。该项目利用钙调素蛋白和肽之间的相互作用，基于一氧化氮合酶的钙调素结合区，开发用于固定肽的最佳程序，开发允许测量动力学和热力学参数的测定，并优化和测量传感器性能。该项目的成功完成将使Nicoya和加拿大处于有利地位，将这种新型传感器平台的应用扩展到研究市场。

This project is intended to solve the specific problem of developing novel peptide-protein interaction assay protocols for the surface plasmon resonance based interaction analysis system from Nicoya Lifesciences. Nicoya is interested in expanding the applications of their system to the thermodynamic and kinetic analysis of biomolecular interactions for the research market and the Dieckmann laboratory is experienced in using surface based methods for the study of specific protein-peptide interactions. This project utilizes the well characterized interactions between the protein Calmodulin and peptides based on the Calmodulin binding region of nitric oxide synthase to develop optimal procedures for the immobilization of the peptides, to develop assays that allow the measurement of kinetic and thermodynamic parameters and to optimize and measure sensor performance. Successful completion of this project will put Nicoya and Canada in a strong position to expand applications of this novel sensor platform into the research market.