Digital polymerase chain reaction (PCR) and high-capacity multiplex PCR for next-generation sequencing

用于新一代测序的数字聚合酶链式反应 (PCR) 和高容量多重 PCR

• 批准号: 474388-2015
• 负责人: Morin, Ryan
• 金额: $ 9.24万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564954
• 关键词: Digital; polymerase; chain; reaction; PCR

Virtually every cell in all organisms contain one or two copies of their genetic instructions known as the genome. The genome is encoded in DNA in a language known as the genetic code. Accidental changes to certain places in the genome (alterations of its sequence; known as mutations) can contribute to evolution or can have disastrous effects, leading to diseases or death of the organism. The requested instrument allows us to determine whether specific genetic changes have occurred in a cellular population or tissue and to accurately count the number of DNA molecules containing a specific sequence. This process, known as "digital PCR", can also be used to accurately identify the presence of DNA molecules that have been mutated or are variable ("polymorphic") in a population. The system we have requested also facilitates efficient reading of small regions of a genome using a technique known as DNA sequencing. This system will be used to study the genetic dynamics in environmental pathogens such as bacteria and disease-carrying insects as well as model organisms. It will also be used to study genetic conditions in mammals including cancers that commonly affect companion dogs.

几乎所有生物体的每个细胞都包含一个或两个被称为基因组的遗传指令的副本。基因组在DNA中以一种称为遗传密码的语言编码。基因组中某些位置的意外变化（其序列的改变;称为突变）可能有助于进化或可能产生灾难性的影响，导致生物体的疾病或死亡。所要求的仪器使我们能够确定细胞群体或组织中是否发生了特定的遗传变化，并准确计数包含特定序列的DNA分子的数量。这个过程，被称为“数字PCR”，也可以用来准确地识别已经突变的DNA分子的存在或在群体中是可变的（“多态性”）。我们所要求的系统还有助于使用称为DNA测序的技术有效地阅读基因组的小区域。该系统将用于研究环境病原体（如细菌和携带疾病的昆虫）以及模式生物的遗传动力学。它还将用于研究哺乳动物的遗传条件，包括通常影响伴侣犬的癌症。