In vivo cell signaling dynamics in spinal cord patterning

多细胞生物的发展依赖于广泛的细胞间通讯，这种通讯是由少量保守的细胞信号通路介导的。过去30年的研究揭示了不同的信号成分和信号策略。然而，关于细胞信号的经典工作要么是在活体外的组织培养中进行的，要么是不得不依赖于解释静态发育时间点的事件(快照)。这些方法未能捕捉到发育的高度动态本质，在这种情况下，细胞与许多其他细胞相互作用，并随着时间的推移遇到各种信号分子，特别是因为组织形态发生往往会导致细胞的广泛运动。因此，开发体内系统以了解细胞如何正确整合多种信号，然后在发育中的组织中产生特定和适当的细胞反应是至关重要的。

Development of multicellular organisms relies on extensive cell-to-cell communication, which is mediated by a small number of conserved cell signaling pathways. Studies in the past 30 years have revealed distinct signaling components and signaling strategies. However, classical work on cell signaling has been either carried out in tissue culture outside of living organisms or has had to rely on interpreting events at static developmental time-points (snapshots). These approaches fail to capture the highly dynamic nature of development, where cells interact with many other cells and encounter a variety of signaling molecules over time, especially since tissue morphogenesis often results in extensive cell movements. It is therefore essential to develop in vivo systems to understand how cells can correctly integrate multiple signals and then produce a specific and appropriate cellular response in developing tissues.