Genetic and biochemical methods for identifying protein interaction motifs that confer nuclear localization

用于识别赋予核定位的蛋白质相互作用基序的遗传和生化方法

• 批准号: RGPIN-2015-06758
• 负责人: Mymryk, Joe
• 金额: $ 2.48万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591270
• 关键词: Genetic; biochemical; methods; identifying; protein

Many proteins are precisely targeted to particular sub-cellular compartments by specific localization signals. Nuclear import of proteins is typically mediated by interaction with soluble cytosolic receptor proteins via nuclear localization signals (NLSs). Canonical NLSs contain either a short stretch of basic amino acids (monopartite) or two closely spaced short stretches of basic amino acids (bipartite). Despite the short length of canonical NLSs, which are as little as 4 or 5 amino acids in length, they make high affinity interactions in the cytosol with members of the importin family of NLS receptors (also known as karyopherins). Importins function as carriers to direct localization of the interacting cargo to the nucleus. The interaction of cargo/carrier complexes with components of the nuclear pore complex leads to nuclear import.

许多蛋白质通过特定的定位信号精确地靶向特定的亚细胞区室。 蛋白质的核输入通常通过经由核定位信号（NLS）与可溶性胞质受体蛋白的相互作用来介导。 典型的NLS包含一段短的碱性氨基酸（单组分）或两段间隔很近的短碱性氨基酸（双组分）。 尽管典型NLS的长度短，其长度少至4或5个氨基酸，但它们在胞质溶胶中与NLS受体的输入蛋白家族（也称为核转运蛋白）的成员进行高亲和力相互作用。 输入蛋白作为载体发挥作用，将相互作用的货物直接定位到细胞核。 货物/载体复合物与核孔复合物的组分的相互作用导致核输入。