Delimitation of protein function & interaction networks through peptide arrays

蛋白质功能的界定

• 批准号: RTI-2016-00237
• 负责人: Li, ShawnShunCheng
• 金额: $ 10.93万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591382
• 关键词: Delimitation; protein; function; interaction; networks

A critical bottleneck in the path of translating a genomic discovery to medicine is that it is not apparent or easily deducible how gene mutations result in aberrant changes in protein function and the accompanying protein-protein interaction (PPI) or regulatory network. To overcome this limitation, it is critical to devise facile and robust strategies to decode the functional proteome that drives disease initiation and progression. Peptide array is a powerful method by which to interrogate the function and connectivity of proteins in health and disease. The ability to synthesize thousands of peptides directly on cellulose membranes has brought transformative changes in proteomics. Dr. Li and the co-applicants, Drs. Litchfied, O’Donoghue and Shilton, have been conducting cutting edge research in proteomics and chemical biology which is dependent, in large part, on peptide arrays and soluble peptides. It is essential that this group of productive and innovative scientists to have access to the state-of-the-art instrumentation in peptide and peptide array synthesis in order for them to conduct cutting edge research in proteomics and related fields.

将基因组发现转化为医学的关键瓶颈是基因突变如何导致蛋白质功能和伴随的蛋白质-蛋白质相互作用（PPI）或调控网络的异常变化并不明显或不容易推断。为了克服这一局限性，关键是要设计出简单而强大的策略来解码驱动疾病发生和发展的功能蛋白质组。肽阵列是一种强有力的方法，通过它来询问蛋白质在健康和疾病中的功能和连接。直接在纤维素膜上合成数千种肽的能力给蛋白质组学带来了变革性的变化。Li博士和共同申请人Litchfied博士、O'Donoghue博士和Shilton博士一直在蛋白质组学和化学生物学领域进行前沿研究，这在很大程度上依赖于肽阵列和可溶性肽。至关重要的是，这群富有成效和创新精神的科学家能够获得肽和肽阵列合成中最先进的仪器，以便他们在蛋白质组学和相关领域进行尖端研究。