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Development of a Capillary Electrophoresis (CE)-based Integrated Analytical Platform for Low Abundance Proteins

Development of a Capillary Electrophoresis (CE)-based Integrated Analytical Platform for Low Abundance Proteins
开发基于毛细管电泳 (CE) 的低丰度蛋白质集成分析平台
批准号:
250020-2012
负责人:
Yeung, Ken
金额:
$2.55万
依托单位国家:
加拿大
项目类别:
Discovery Grants Program - Individual
财政年份:
2016
资助国家:
加拿大
项目状态:
已结题
起止时间:
2016-01-01 至 2017-12-31

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中文摘要
翻译
蛋白质组学是对生物系统中蛋白质的大规模研究,导致了对人类生理学和药物发现进展的理解的范式转变。质谱(MS)是蛋白质组学中的重要工具。在过去的十年中,MS的进步导致了速度,可靠性和最重要的灵敏度的显着改善。灵敏度对于发现低丰度蛋白质至关重要,这些蛋白质可能是关键的药物靶点。虽然今天的MS技术能够检测非常少量的蛋白质,但大多数现有的样品制备程序需要大量的蛋白质。为了充分利用先进MS的能力,以非常小的体积处理少量蛋白质是必不可少的。
英文摘要
Proteomics, a large-scale study of proteins in biological systems, led to a paradigm shift in the understanding of human physiology and drug discovery advances. Mass spectrometry (MS) is an essential tool in proteomics. Advancements in MS over the past decade have resulted in dramatic improvements in speed, reliability and most importantly, sensitivity. Sensitivity is vital in the discovery of low abundance proteins that can be crucial drug targets. While today's MS technology is capable of detecting very small quantities of proteins, most of the currently existing sample preparation procedures require much larger amount of proteins. To fully utilize the power of advanced MS, handling of small quantities of proteins in very small volumes is essential.
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Imaging Mass Spectrometry of Biological Tissues
  • 批准号:
    RGPIN-2017-05584
  • 项目类别:
    Discovery Grants Program - Individual
  • 资助金额:
    $4.08万
  • 财政年份:
    2021
  • 负责人:
    Yeung, Ken
  • 依托单位:
Imaging Mass Spectrometry of Biological Tissues
  • 批准号:
    RGPIN-2017-05584
  • 项目类别:
    Discovery Grants Program - Individual
  • 资助金额:
    $2.04万
  • 财政年份:
    2020
  • 负责人:
    Yeung, Ken
  • 依托单位:
Imaging Mass Spectrometry of Biological Tissues
  • 批准号:
    RGPIN-2017-05584
  • 项目类别:
    Discovery Grants Program - Individual
  • 资助金额:
    $2.04万
  • 财政年份:
    2019
  • 负责人:
    Yeung, Ken
  • 依托单位:
Imaging Mass Spectrometry of Biological Tissues
  • 批准号:
    RGPIN-2017-05584
  • 项目类别:
    Discovery Grants Program - Individual
  • 资助金额:
    $2.04万
  • 财政年份:
    2018
  • 负责人:
    Yeung, Ken
  • 依托单位:
海外基金