Development of a Capillary Electrophoresis (CE)-based Integrated Analytical Platform for Low Abundance Proteins

开发基于毛细管电泳 (CE) 的低丰度蛋白质集成分析平台

• 批准号: 250020-2012
• 负责人: Yeung, Ken
• 金额: $ 2.55万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=594379
• 关键词: Development; Capillary; Electrophoresis; CE; based

Proteomics, a large-scale study of proteins in biological systems, led to a paradigm shift in the understanding of human physiology and drug discovery advances. Mass spectrometry (MS) is an essential tool in proteomics. Advancements in MS over the past decade have resulted in dramatic improvements in speed, reliability and most importantly, sensitivity. Sensitivity is vital in the discovery of low abundance proteins that can be crucial drug targets. While today's MS technology is capable of detecting very small quantities of proteins, most of the currently existing sample preparation procedures require much larger amount of proteins. To fully utilize the power of advanced MS, handling of small quantities of proteins in very small volumes is essential.

蛋白质组学是一项对生物系统中蛋白质的大规模研究，它导致了对人类生理学和药物发现进展的理解的范式转变。质谱（MS）是蛋白质组学研究的重要工具。在过去的十年中，MS的进步导致了速度、可靠性和最重要的灵敏度的显著提高。在发现可能成为关键药物靶点的低丰度蛋白质时，灵敏度至关重要。虽然今天的质谱技术能够检测非常少量的蛋白质，但目前大多数现有的样品制备程序需要大量的蛋白质。为了充分利用先进质谱的力量，处理非常小体积的少量蛋白质是必不可少的。