Engineering of a Perfusion Chamber for High-Density Cultures of Endocrine Pancreatic Islets for Insulin Production

用于胰岛素生产的内分泌胰岛高密度培养灌注室的工程

• 批准号: 250296-2012
• 负责人: Vermette, Patrick
• 金额: $ 2.48万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=594433
• 关键词: Engineering; Perfusion; Chamber; Density; Cultures

In 2010, the International Diabetes Federation estimated that 285 millions people worldwide have diabetes. This includes more than three millions Canadians, and these numbers are increasing dramatically owing to a number of factors. The financial burden on people and on the Canadian healthcare system is enormous ($13 billion/year). The loss of insulin-producing cells (beta cells) has been observed in both type 1 and type 2 diabetes. Pancreatic islet cell transplantation can restore the required mass of functional beta-cells to achieve normoglycemia in diabetics. However, this strategy has encountered many problems including the unavailability of a standard islet transplant protocol, insufficient islet yields after isolation and beta-cell apoptosis following transplantation. The LONG-TERM PROGRAM OBJECTIVE is to develop a chamber to support a high-density culture of endocrine pancreatic islets for insulin production.

2010年，国际糖尿病联合会估计全世界有2.85亿人患有糖尿病。这包括300多万加拿大人，由于一些因素，这些数字正在急剧增加。对人民和加拿大医疗保健系统的财政负担是巨大的（130亿美元/年）。在1型和2型糖尿病中均观察到胰岛素产生细胞（β细胞）的损失。胰岛细胞移植可以恢复所需的功能性β细胞的质量，以实现糖尿病患者的正常血糖。然而，这种策略遇到了许多问题，包括标准胰岛移植方案的不可用性、分离后胰岛产量不足以及移植后β细胞凋亡。长期目标是开发一种支持高密度培养内分泌胰岛以产生胰岛素的培养箱。