Plant endophytes: bacterial and fungal associations that improve plant health, facilitate soil nutrient transformations and sustain soil ecosystems

我的研究调查了植物和微生物之间的有益关联，以了解这些关系如何影响植物健康、养分循环和生态系统的可持续性。这项工作的一个主要焦点是研究植物内生菌。内生菌是与植物形成有益结合的细菌和真菌，生活在植物组织中，如根、茎或叶，不会对植物造成伤害。许多不同类型的细菌和真菌与植物形成内生联系，但人们对这些联系的多样性和特异性知之甚少。了解植物内生菌很重要，因为这些细菌或真菌可以帮助植物生长，克服高温和干旱等压力，甚至净化环境。因此，我工作的一个主要重点是在一系列植物物种中发现内生菌(主要是根部内生菌)，从本地植被到农业作物，再到用于开垦土地的植物。了解哪些细菌或真菌形成了内生联系，这将使人们有可能了解这些联系是否是特定微生物和植物之间的独特联系。它还将使我们能够评估植物内生菌的功能作用。例如，我过去的一些研究表明，某些内生真菌可以提高小麦等作物的耐热性和抗旱性。 我的研究将集中于以下问题：1)在用于开垦的农作物和植物中存在哪些独特的植物内生菌？2)什么土壤或植物因素影响和调节植物根部内生细菌的联合？3)细菌根部内生菌是否与真菌根部内生菌相互作用，如果是的话，这些相互作用能否被利用来影响植物的健康和生长？4)内生菌对植物微生物群的相对重要性是什么？5)植物内生菌能否被用作促进作物生长或帮助土地复垦活动的生物技术工具？总体而言，这些研究将促进我们对植物与内生根细菌或真菌之间的特定联系如何影响植物生长的理解，从而导致健康和可持续的土壤生态系统。我的研究很重要，因为新的植物内生菌的发现提供了一个机会来识别独特的植物-微生物协会，这些协会可以通过增加粮食产量或帮助清洁环境来造福加拿大社会。这项研究的另一个关键成果是培养了4名博士和2名硕士学生。

My research investigates beneficial associations between plants and microorganisms so as to understand how these relationships affect plant health, nutrient cycling and ecosystem sustainability. A primary focus of this work is to study plant endophytes. Endophytes are bacteria and fungi that form beneficial associations with plants, living inside plant tissues such as roots or stems or leaves, without causing harm to the plant. Many different types of bacteria and fungi form endophytic associations with plants, but little is know about the diversity and specificity of these relationships. It is important to understand plant endophytes because these bacteria or fungi can help plants grow, over come stresses like heat and drought, and even clean up the environment. Thus a main focus of my work is on the discovery and characterization of endophytes (primarily root endophytes) in a range of plants species from native vegetation to agriculture crops to plants used for land reclamation. Understanding which bacteria or fungi form endophytic associations will make it possible to learn if these are unique associations between specific microbe and plants. It will also allow us to assess the functional role of endophytes in plants. For example, some of my past research demonstrated that certain endophytic fungi improve heat and drought tolerance of crops like wheat. My research will focus on the following questions: 1) What unique plant endophytes exist in agricultural crops and plants used for land reclamation? 2) What soil or plant factors influence and regulate endophytic root bacteria associations with plants? 3) Do bacterial root endophytes interact with fungal root endophytes, and if so can these interactions be exploited to influence plant health and growth? 4) What is the relative importance of endophytes to the plant microbiome? 5) Can plant endophytes be utilized as biotechnology tools to increase crop growth or to help land reclamation activities? Collectively these studies will advance our understanding of how specific associations between plants and endophytic root bacteria or fungi affect plant growth, thus leading to healthy and sustainable soil ecosystems. My research is important as discovery of new plant endophytes offers an opportunity to identify unique plant-microbe associations that can be used to benefit Canadian society by increasing food production or help to clean up the environment. Another key outcome of this research is the training of 4PhD and 2 MSc students.