Environmental regulation of epigenetics in aquatic teleosts

水生生态系统目前正面临着无数的非生物（如温度、氧气供应、酸化）和人为（如药物污染物、金属、农业径流）影响，这些影响对环境和依赖环境的生物的健康产生重大影响，这些威胁为数众多，并非独立存在，而是累积起来改变我们的生态系统。然而，我们确保生态系统可持续性的能力受到我们理解和预测这些威胁所产生的各种压力如何相互作用以引起变化的能力的限制。虽然个别的压力对水生生态系统造成了记录在案的问题，但令人关切的是，这些影响的组合将对这些环境，更具体地说，对生活在其中的水生生物造成更重大的挑战。许多法规、政策、监控和补救计划都是基于对单一因素的理解（例如，温度或特定污染物），因此不充分。正是在此基础上，我的研究计划是基于-获得在环境中发现的多种，混合的压力和对水生物种，特别是硬骨鱼的影响的基本了解。通过采取综合方法，我的研究计划将跨越生物组织的各个层面，从DNA调控到整个动物生理学，再到预测混合压力源对物种健康和丰度的影响。我的研究将集中在与气候变化（温度/缺氧）相关的非生物因素，以及新兴的有机污染物（药物），这些污染物已被证明针对许多鱼类。这种新的方法将研究环境如何影响表观遗传调控，以及这些变化的下游功能后果。表观遗传学的定义是检查DNA序列变化以外的变异遗传。通过DNA甲基化、组蛋白修饰和非编码RNA的表观遗传调控可能会深刻地改变基因表达的转录和翻译效应，现在很清楚，这些机制受到自然和人为环境压力的影响，作为基因组和环境之间的界面。我不仅将比较和对比的表观遗传调控的硬骨鱼的寿命内，但也检查内和代际遗传的表观遗传机制从母亲和父亲的来源，这将突出后代的易感性从以前的环境压力暴露。专注于模型基因组（斑马鱼）和生理（虹鳟鱼）物种，我将大大提高我们的理解复杂的生态系统相互作用，使用综合多应激实验。

Aquatic ecosystems are currently facing a myriad of abiotic (e.g. temperature, oxygen availability, acidification) and anthropogenic (e.g. pharmaceutical contaminants, metals, agricultural runoff) influences that have a significant impact on the health of the environment and the organisms that depend upon it. These threats are numerous and are not independent, but work cumulatively to alter our ecosystems. However, our ability to ensure ecosystem sustainability is limited by our capacity to understand and predict how the various stressors resulting from these threats interact to cause change. While individual stressors poses documented problems for aquatic ecosystems, the concern is that combinations of these effects will create even more significant challenges to these environments and, more specifically, aquatic organisms living in them. Many regulations, policies, monitoring and remediation programs are based on an understanding of only single factors (e.g., temperature or one specific pollutant) and are therefore inadequate. It is upon this that my research program is based – to gain a fundamental understanding of multiple, mixed stressors found within the environment and the impact upon aquatic species, specifically teleost fishes. By taking an integrative approach, my research program will cross all levels of biological organization, from DNA regulation to whole animal physiology towards making predictions regarding the impact of mixed stressors on species health and abundance. My research will focus on abiotic factors associated with climate change (temperature/hypoxia) in combination with emerging organic pollutants (pharmaceuticals), which have been shown to target numerous fish species. This novel approach will examine how the environment affects epigenetic regulation, and the downstream, functional consequences of these changes. Epigenetics is defined by examining the inheritance of variation beyond changes in DNA sequence. Epigenetic regulation, through DNA methylation, histone modification, and non-coding RNA may profoundly alter transcriptional and translational effects of gene expression, and it is now clear that these mechanisms are influenced by environmental stressors, both natural and anthropogenic, acting as the interface between the genome and the environment. Not only will I compare and contrast epigenetic regulation within the lifespan of teleosts, but also examine the intra- and inter-generational inheritance of epigenetic mechanisms from maternal and paternal sources, which will highlight the susceptibility of future generations from prior environmental stress exposure. Focusing on both model genomic (zebrafish) and physiological (rainbow trout) species, I will significantly advance our understanding of complex ecosystem interactions using integrated multi-stressor experimentation.