High resolution characterization of macromolecular structure and dynamics

基金 本NSERC研究工具和仪器(第1类)拨款申请 应用程序将用于购买最先进的和新的市场规模 排斥色谱多角度静态光散射(SEC-MALS)系统 并升级多用户分析超速离心机系统。两个系统 是相辅相成的，并允许详细的生物物理 通过获得准确的蛋白质和其他生物分子的表征 样品的相对分子质量和低聚物态。这些仪器允许 用于直接检测蛋白质构象变化，蛋白质动力学， 蛋白质的稳定性，以及蛋白质与配体之间的相互作用 申请。这些仪器对我们开展的研究至关重要 团队致力于在全球范围内表征蛋白质的结构和功能 不同的模型系统。 这个 团队由8名首席调查员组成，他们曾共同工作过， 在几个不同的项目上一起出版。此外，这些仪器 ，供市民大众使用。 蛋白质生物物理中心位于华盛顿大学生物化学系 多伦多大学。除了我们小组的成员，学生和 来自大约40个其他小组的博士后研究员经常使用生物物理 中心的仪器，其中包括高端荧光计，平板阅读器， 圆二色光谱偏振仪，质谱仪，等温 滴定量热计，以及其他仪器。这些研究小组的研究发表在 蛋白质折叠和相互作用动力学的一般领域。 拥有 作为蛋白质生物物理中心的一部分，这些最先进的系统将 确保为学员提供丰富的研究环境。中锋将吸引 顶尖本科生和研究生以及博士后研究员将拥有 访问一系列全面的最新生物物理技术 完全集成和管理的环境。这将是非常重要的 使我们的团队保持在蛋白质生物物理学领域的前沿。

Funds requested in this NSERC Research Tools and Instruments (Category 1) grant application will be used to purchase a state-of-the-art and new-to-market Size Exclusion Chromatography Multi-Angle Static Light Scattering (SEC-MALS) system and to upgrade a multiuser analytical ultracentrifugation system. Both systems are complementary to each other and allow the detailed biophysical characterization of proteins and other biomolecules by obtaining accurate molecular weights and oligomeric states of the samples. The instruments allow for the direct detection of protein conformational changes, protein dynamics, protein stability, and protein-ligand interactions among many other applications. These instruments are critical to the research carried out by our team aimed globally at characterizing protein structure and function in different model systems. The team consists of eight Principal Investigators who have worked together and published together on several different projects. In addition, the instruments will be made available for use to the community in general by being placed at the Protein Biophysics Center located in the Department of Biochemistry at the University of Toronto. In addition to members of our groups, students and postdoctoral fellows from about 40 other groups routinely use the biophysical instruments at the Center, which houses a high end fluorometer, plate reader, circular dichroism spectropolarimeter, a mass spectrometer, isothermal titration calorimeter, among other instruments. Research by those groups is in the general area of protein folding and interaction dynamics. Having these state-of-the-art systems as part of the Protein Biophysics Center will ensure a rich research environment for our trainees. The center will attract top undergraduate and graduate students and post-doctoral fellows who will have access to a comprehensive array of the latest biophysical technologies in a totally integrated and managed environment. This will be of great importance to keep our groups at the forefront of the field of protein biophysics.