The Biophysics of Developmentally Regulated Gene Transcription

发育调控基因转录的生物物理学

• 批准号: RGPIN-2017-04133
• 负责人: McGhee, James
• 金额: $ 2.04万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629024
• 关键词: Biophysics; Developmentally; Regulated; Gene; Transcription

Initiation of gene transcription is arguably the most influential control point in the development of a multicellular organism. In the simplest version of gene activation, a specific transcription factor binds to a short DNA sequence (6-12 bps) in the control region of a target gene and then somehow recruits the vast biochemical machinery of RNA Polymerase II and its auxiliary proteins, thereby initiating transcription. All of this occurs in an environment of “chromatin”, featuring possible cofactors, differentially modified histones and potentially positioned nucleosomes. We will focus on the all-important early step of gene activation in which a single controlling transcription factor interacts with its binding site in the regulatory region of a target gene. Our goal is to relate precise biophysical measurements made with purified components

基因转录的起始可以说是多细胞生物体发育中最有影响力的控制点。在基因激活的最简单版本中，特定的转录因子与靶基因控制区的短DNA序列（6-12 bp）结合，然后以某种方式招募RNA聚合酶II及其辅助蛋白的巨大生化机制，从而启动转录。所有这些都发生在“染色质”的环境中，其特征在于可能的辅因子，差异修饰的组蛋白和潜在定位的核小体。我们将集中在基因激活的所有重要的早期步骤，其中一个单一的控制转录因子与其在靶基因的调控区的结合位点相互作用。我们的目标是与纯化成分进行精确的生物物理测量