Regulation of mRNA Translation During Cellular Stress and Apoptosis

细胞应激和细胞凋亡过程中 mRNA 翻译的调节

• 批准号: RGPIN-2017-05463
• 负责人: Thakor, Nehalkumar
• 金额: $ 1.89万
• 依托单位: University of Lethbridge
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629510
• 关键词: Regulation; mRNA; Translation; During; Cellular

The process of gene expression is tightly regulated at various levels including transcriptional, translational and post-translational steps. The regulation of gene expression at the translational level allows cells to rapidly reprogram protein output in response to stress stimuli. During stress conditions, global mRNA translation (canonical translation) is reduced due to modification of key eukaryotic translation initiation factors (eIFs). However, the alternative means of translation initiation (non-canonical translation) allow production of proteins that are required to manage stress conditions. For example, the production of both pro-survival and pro-death proteins is regulated via non-canonical translation mechanisms. Thus, the non-canonical translation is considered to play a central role during cellular stress.

基因表达过程在转录、翻译和翻译后等多个水平受到严格调控。在翻译水平上对基因表达的调节允许细胞响应于应激刺激而快速重编程蛋白质输出。在应激条件下，由于关键真核翻译起始因子（eIF）的修饰，整体mRNA翻译（规范翻译）减少。然而，翻译起始的替代手段（非规范翻译）允许产生管理应激条件所需的蛋白质。例如，促存活蛋白和促死亡蛋白的产生通过非经典翻译机制来调节。因此，非规范翻译被认为在细胞应激期间发挥核心作用。