Mechanisms of selective host gene translation regulation in picornavirus infection

小核糖核酸病毒感染中选择性宿主基因翻译调控机制

• 批准号: RGPIN-2017-05612
• 负责人: Yang, Decheng
• 金额: $ 2.48万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629569
• 关键词: Mechanisms; selective; host; gene; translation

Picornaviral infection causes global shutdown of host gene translation but still maintains essential translation of certain host genes supporting viral replication. In the search for such host cellular mRNAs, the 5’TOP (terminal oligopyrimidine tract) mRNAs are candidates of interest. The 5’TOP motif is defined by a 7-methyl-G cap followed by a C and then a stretch of 4-15 CU at the 5’ end of mRNA. The 5’TOP mRNAs (~30% of total cellular mRNAs) encode proteins of translational machinery such as ribosomal proteins and translation initiation and elongation factors. The presence of the 5’TOP motif within these mRNAs has previously been shown to cause its translation

小核糖核酸病毒感染导致宿主基因翻译的全球关闭，但仍维持支持病毒复制的某些宿主基因的必要翻译。在寻找这样的宿主细胞mRNAs的过程中，5‘端(末端寡嘧啶束)mRNAs是感兴趣的候选者。5‘顶端基序由7-甲基-G帽和C组成，然后在mRNA的5’端延伸4-15CU。5‘端的mRNAs(约占细胞总mRNAs的30%)编码翻译机制的蛋白质，如核糖体蛋白、翻译起始和延伸因子等。这些mRNAs中5‘顶端基序的存在已被证明是导致其翻译的原因