Novel platforms for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cattle.

用于检测鸟分枝杆菌亚种的新平台。

• 批准号: RGPIN-2015-04607
• 负责人: DeBuck, Jeroen
• 金额: $ 1.75万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630545
• 关键词: Novel; platforms; detection; Mycobacterium; avium

Johne’s disease (JD), a debilitating chronic enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map), is one of the main health issues in the dairy industry, resulting in significant economical impact. Prevention and control programs are severely hampered by the poor performance of current diagnostic tests, especially in the early stages of the infection. They fail to reliably detect infected animals before they can spread the disease. Current ELISAs for antibody (Ab) testing have crude antigen (Ag) mixtures that are not optimized for early detection. Fecal shedding and Ab responses correlate poorly, but combined testing for these increases testing sensitivity.

约翰氏病（JD）是由鸟分枝杆菌（Mycobacterium avium subsp.）副结核病（MAP）是乳制品行业的主要健康问题之一，导致显著的经济影响。预防和控制计划受到当前诊断测试性能不佳的严重阻碍，特别是在感染的早期阶段。他们无法在感染动物传播疾病之前可靠地检测到它们。目前用于抗体（Ab）测试的ELISA具有未针对早期检测优化的粗抗原（Ag）混合物。粪便脱落和抗体反应相关性较差，但这些组合测试增加了测试的灵敏度。