New Dimensions and Domains for Fluorescence Fluctuation Imaging Methods

荧光波动成像方法的新维度和新领域

• 批准号: RGPIN-2017-05005
• 负责人: Wiseman, Paul
• 金额: $ 7.72万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=632367
• 关键词: New; Dimensions; Domains; Fluorescence; Fluctuation

In the past decade there has been a revolution in light microscopy recognized with the awarding of the 2014 Chemistry Nobel Prize to Betzig, Moerner and Hell for the development of super resolved fluorescence microscopy. This technical renaissance has witnessed tremendous advances in both super-resolution fluorescence microscopy and in single plane illumination microscopy (SPIM) also commonly known as light sheet microscopy. As was the case at the advent of light microscopy in the 17th century, these new technological leaps offer great promise for biomedical research science on living cells and organisms. However, many forms of super-resolution microscopy are based on iterative molecular localization leading to a kinetic bottleneck where temporal resolution is sacrificed to gain spatial resolution.

在过去的十年中，光学显微镜发生了一场革命，2014年诺贝尔化学奖授予Betzig，Moerner和Hell，以表彰他们在超分辨荧光显微镜方面的发展。这一技术复兴见证了超分辨率荧光显微镜和单面照明显微镜（SPIM）（通常也称为光片显微镜）的巨大进步。正如光学显微镜在17世纪出现时的情况一样，这些新的技术飞跃为活细胞和生物体的生物医学研究科学提供了巨大的希望。然而，许多形式的超分辨率显微镜是基于迭代的分子定位，导致动力学瓶颈，其中牺牲时间分辨率以获得空间分辨率。