Nanopatterning of advanced research tools to harness the mechanobiology of cell-matrix interaction for stem cell expansion

先进研究工具的纳米图案利用细胞-基质相互作用的机械生物学来进行干细胞扩增

• 批准号: RGPIN-2016-04043
• 负责人: Yim, Evelyn
• 金额: $ 2.04万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=638079
• 关键词: Nanopatterning; advanced; research; tools; harness

Pluripotent stem cells (PSC), which can give rise to different cell types of all three germ layers, are excellent models to study tissue development and lineage commitment in vitro. While conventional culture conditions for PSCs focus on biochemical definition, biophysical cues that are present in the stem cell niche are not incorporated in vitro. Our previous studies have demonstrated the significant influence of topography on stem cell fate determination through the involvement and restructuring of focal adhesion. Yet the biophysical regulation of PSC pluripotency maintenance and lineage commitment in the in vitro niche remains a mystery. We hypothesize that 1) biophysical cues such as nanotopography regulate pluripotency maintenance via a focal adhesion-signaling pathway; 2) a suitable biophysical environment and biochemical factor can work synergistically to enhance human PSC maintenance; and 3) advanced nanofabrication techniques can fabricate nanopatterned tools and 3D bioreactors for stem cell expansion.

多能干细胞（PSC）可以产生所有三个胚层的不同细胞类型，是体外研究组织发育和谱系定型的极好模型。虽然PSC的常规培养条件集中在生物化学定义上，但在干细胞龛中存在的生物物理线索不在体外并入。我们以前的研究已经证明了地形通过参与和重组粘着斑对干细胞命运的决定有重要影响。然而，PSC多能性维持和谱系定型在体外生态位中的生物物理调节仍然是一个谜。我们假设：1）生物物理学线索，如纳米形貌，通过局部粘附信号传导途径调节多能性维持; 2）合适的生物物理学环境和生化因子可以协同作用，以增强人类PSC维持; 3）先进的纳米制造技术可以制造纳米图案化工具和3D生物反应器用于干细胞扩增。