Extraction of rosavin, rosin and rosarin from Rhodiola rosea roots using supercritical CO2

红景天是一种草本植物，生长在北极地区和阿尔伯塔，传统上用于药用。各种研究已经证明了红景天在健康的几个领域的生物活性，包括压力和癌症等。在治疗效果方面感兴趣的是其rosavin化合物。采用不同的溶剂提取方法从红景天根中提取rosavin。然而，对于这些现有的提取物，所得rosavin的量和纯度在质量和产率方面仍然是一个重大的挑战。此外，关于这种生物活性复合物的therosin和rosarin组分的提取知之甚少，这些生物活性复合物统称为rosavins。本项目的主要目的是利用超临界CO2萃取技术，一种环境友好的“绿色”技术，从St.弗朗西斯草药农场公司提供的红景天根中提取trosavins，即rosavin，松香和rosarin。使用二氧化碳作为溶剂的超临界萃取可以很容易地将溶剂与提取的rosavin，松香和rosarin分离，留下易于表征的产品。超临界萃取的结果将与传统的溶剂萃取在产量和产品质量方面进行比较。高产量的纯rosavin，松香和rosarin将使其进一步商业化，作为加拿大生产的天然健康产品的成分，并将加强加拿大的农业部门。rosavin、rosin和rosarin的成功提取和商业化还具有潜在的社会效益，即为全球受这些健康问题影响的大部分女性和男性提供负担得起的治疗热潮红、压力和抑郁症的治疗。

Rhodiola rosea is an herbaceous plant found in the Arctic region and Alberta that has traditionally been usedfor medicinal purposes. Various studies have demonstrated the bioactivity of Rhodiola rosea in several areas ofhealth, including stress and cancer, among others. Of interest in terms of therapeutic effectiveness are itsrosavin compounds. Different solvent extraction methodologies have been used to isolate rosavin fromRhodiola rosea roots. However, with these existing extracts the amount and purity of the resulting rosavin interms of both quality and yield remain a significant challenge. Also, little is known about the extraction of therosin and rosarin components of this bioactive complex collectively called rosavins. The main objective of thisproject is to use supercritical CO2 extraction, an environmentally-friendly "green" technology, to extractrosavins, i.e. rosavin, rosin and rosarin, from Rhodiola rosea roots provided by St. Francis Herb Farm Inc.Supercritical extraction using carbon dioxide as the solvent allows easy separation of the solvent from theextracted rosavin, rosin, and rosarin, leaving a product that is easy to characterize. Results from supercriticalextraction will be compared to conventional solvent extraction in terms of both yield and product quality. Ahigh yield of pure rosavin, rosin, and rosarin will allow their further commercialization as ingredients of naturalhealth products produced in Canada and will strengthen Canada's agricultural sector. The successful extractionand commercialization of rosavin, rosin, and rosarin also has the social benefit of potentially providing anaffordable treatment for hot flashes, stress, and depression to a large segment of women and men affected bythese health issues worldwide.