Comprehensive assessment of mitochondrial bioenergetics; enabling simultaneous measurements in controlled oxygen environments

线粒体生物能量学综合评估；

• 批准号: RTI-2018-00283
• 负责人: Holloway, Graham
• 金额: $ 5.03万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642189
• 关键词: Comprehensive; assessment; mitochondrial; bioenergetics; enabling

This is a request for a cutting-edge mitochondrial bioenergetics system that can simultaneously determine several aspects of mitochondrial function. Cells consume oxygen while breaking down food to produce energy (ATP). The historical gold-standard for assessing mitochondrial function reflects determining rates of oxygen consumption and this methodology has become the corner-stone of our research programs. However, a major limitation of this approach is that rates of ATP production are not determined, which is the ultimate goal of oxidative phosphorylation. The requested piece of equipment will enable simultaneous assessments of oxygen consumption and ATP production, negating this methodological limitation, while also enabling more diverse and biologically relevant mechanistic studies to be designed.

这是对尖端线粒体生物能量学系统的要求，该系统可以同时确定线粒体功能的几个方面。细胞消耗氧气，同时分解食物产生能量（ATP）。评估线粒体功能的历史黄金标准反映了确定氧消耗率，这种方法已成为我们研究计划的基石。然而，这种方法的一个主要限制是ATP产生的速率不确定，这是氧化磷酸化的最终目标。所要求的设备将能够同时评估氧气消耗和ATP产生，消除这种方法上的限制，同时还能够设计更多样化和生物相关的机制研究。