Detection of EPS

EPS检测

• 批准号: 530812-2018
• 负责人: Blight, Barry
• 金额: $ 1.82万
• 依托单位: University of New Brunswick
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=645632
• 关键词: Detection; EPS

This work will result in the development of a new single molecule fluorescence sensor or extracellular**polymeric substance (EPS) which is a biofilm forming residue that can contain microbial cultures. This work**will have widespread interest in domestic water supplies, industrial wastewater, industrial fluid transfer**processes, and in wastewater treatment. In collaboration with LuminUltra Technologies Ltd, experimental**development and evaluation will be conducted in the research lab of Prof Blight at the University of New**Brunswick and conducted by one graduate student and one research assistant. We will 1) develop a single**molecule fluorescent sensor for EPS; 2) we will test at UNB on comparable materials such as dextrose and**amylopectin; 3) we will then test EPS in the research quarters, on-site at LuminUltra Technologies. There will**be a five-fold benefit to Canada when this collaboration proves successful: 1) Public Safety - benefit of early**detection of biofilm scaffolds that support harmful microbial deposition of domestic water infrastructure; 2)**Infrastructure retention - prevention of MIC and the 'dolomite problem' will extend the lifespan (cost savings)**of domestic water infrastructure; 3) Industry - many industries require microbes for some production process**and/or must prevent microbe growth for others. Our technology will allow rapid detection for biofilm**deposition prevention (safety and cost savings); 4) Science Impact - a product of this type, developed in**Canada, will make a significant impact on profile of Canadian Science; 5) Economy - the global addressable**market for this product is $1.3 billion worldwide, with potential use in any country, and being both developed**and produced in Canada furthers this impact.

这项工作将导致一种新的单分子荧光传感器或细胞外聚合物（EPS）的发展，这是一个生物膜形成残留物，可以包含微生物培养物。这项工作 ** 将在家庭供水，工业废水，工业流体传输 ** 过程和废水处理中产生广泛的兴趣。与LuminUltra Technologies Ltd合作，将在新不伦瑞克大学Blight教授的研究实验室进行实验性开发和评估，并由一名研究生和一名研究助理进行。我们将1）开发EPS的单 ** 分子荧光传感器; 2）我们将在UNB对葡萄糖和 ** 支链淀粉等类似材料进行测试; 3）然后我们将在LuminUltra Technologies的研究区现场测试EPS。如果这项合作取得成功，将为加拿大带来五倍的好处：1）公共安全-有利于及早发现生物膜支架，这种支架支持生活用水基础设施的有害微生物沉积; 2）基础设施保留-防止MIC和“白云石问题”，将延长使用寿命。（节约成本）**; 3）工业-许多工业需要微生物用于某些生产过程 ** 和/或必须防止微生物生长用于其他生产过程。我们的技术将允许快速检测生物膜 ** 沉积预防（4）科学影响-在 ** 加拿大开发的这类产品将对加拿大科学的形象产生重大影响; 5）经济性-该产品的全球可寻址 ** 市场在全球范围内为13亿美元，在任何国家都有潜在用途，而在加拿大开发和生产的产品则进一步扩大了这种影响。