Cannabis Genotyping Pipeline

Greenway温室大麻公司（Greenway）是一家垂直整合的药用大麻生产商，最近从其母公司Sunrite Greenhouses Ltd剥离出来，Sunrite Greenhouses Ltd拥有超过45年的商业园艺经验和超过400万平方英尺的水培生产面积（50 **万平方英尺）。其中有1英尺是献给格林威的)。绿威还拥有多样化和独特的种质资源**。种子和组织)资源，包括由专业育种者合法赠送的100种高价值大麻品种的种子。尽管Greenway拥有丰富的园艺专业知识和基础设施，但它缺乏遗传学研究能力，因此寻求一个学术合作伙伴来合作开发一种新的、快速的系统来对其种质进行遗传表征。这将使Greenway能够合理地选择其菌株的一个子集进行进一步的开发，繁殖和商业化。这也将为关键性状的遗传作图、品种保护和质量保证奠定基础。为了实现开发一种快速的、基于DNA的方法来定义大麻菌株之间的亲缘关系的合作目标，我们将利用已经在公共领域的1300个大麻菌株的DNA序列信息，并确定一组384个遗传标记（snp，单核苷酸多态性），这些标记将用于通过测序进行基因分型的分析，以产生每个菌株的遗传指纹。这些遗传指纹将从100个高价值菌株和已知菌株中获得，以鉴定相关种质的群集。这些集群还将与收集到的关于每种菌株生长和（未来）化学性质的信息进行比较。信息标记的最小数量将**确定，以进一步简化未来的方案。这些结果将构成未来合作的基础，包括开发多样化的育种和绘制种群。

Greenway Greenhouse Cannabis Corporation (Greenway) is a vertically integrated medicinal cannabis**producer, recently spun off from its parent company, Sunrite Greenhouses Ltd., which has over 45 years of**experience in commercial horticulture and over 4 million square feet of hydroponic production area (0.5**million sq. ft. of this is dedicated to Greenway). Greenway also has access to diverse and unique germplasm**(i.e. seed and tissue) resources, including a collection of 100 high-value cannabis strains gifted legally as seeds**by an expert breeder. Although Greenway has substantial horticultural expertise and infrastructure, it lacks**research capacity in genetics and therefore seeks an academic partner to collaborate on development of a novel,**rapid system for genetically characterizing its germplasm. This will allow Greenway to rationally select a**subset of its strains for further development, breeding, and commercialization. This will also lay a foundation**for genetic mapping of key traits, variety protection, and quality assurance.**To accomplish the collaborative objective of developing a rapid, DNA-based method for defining the**relatedness of cannabis strains, we will leverage DNA sequence information already in the public domain for**1300 cannabis strains, and identify a set of 384 genetic markers (SNPs, single-nucleotide polymorphisms) that**will be used in a genotyping-by-sequencing assay to produce genetic fingerprints for each strain. These genetic**fingerprints will be obtained for the 100 high-value strains and from known strains, to identify clusters of**related germplasm. These clusters will also be compared to information collected about the growth and (in the**future) the chemical properties of each strain. The minimum number of informative markers will be**determined to further streamline the protocol in the future. These results will form the basis of future**collaborations including development of diverse breeding and mapping populations.