Mechanistic Studies of Amino Acid Homeostasis and Organellar Crosstalk by GATOR1.

GATOR1 的氨基酸稳态和细胞器串扰的机制研究。

• 批准号: RGPIN-2018-06227
• 负责人: Dutchak, Paul
• 金额: $ 3.42万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=649824
• 关键词: Mechanistic; Studies; Amino; Acid; Homeostasis

All living organisms have developed intricate regulatory mechanisms to help maintain their viability during periods of nutrient deprivation or starvation. The central goal of the lab is to understand how metabolic changes can influence the biological functions of cells and determine how cells cope with nutritional deficiencies. To this end, we are investigating the biological function of an evolutionarily conserved amino acid-“sensing” protein complex that is present from yeast to humans. Through unknown mechanisms, this complex can sense the abundance of amino acids inside the cell and orchestrate cellular programs that control amino acid incorporation into proteins or conversion into other biomolecules. Intriguingly, we have found that inhibiting this amino acid sensor can also change how cells process carbohydrates, like glucose. Our studies aim to determine how cells integrate the utilization of these fundamental metabolites and define new mechanisms by which nutrient queues influence cellular communication programs to maintain a metabolic balance. We hope these studies will provide novel insights into important regulatory mechanisms of metabolism, and develop new ways to target metabolic changes in cells.

所有的生物体都发展了复杂的调节机制，以帮助在营养缺乏或饥饿期间维持其生存能力。该实验室的中心目标是了解代谢变化如何影响细胞的生物学功能，并确定细胞如何科普营养缺乏。为此，我们正在研究一种进化上保守的氨基酸-“传感”蛋白复合物的生物学功能，该复合物存在于酵母和人类之间。 通过未知的机制，这种复合物可以感知细胞内丰富的氨基酸，并协调控制氨基酸掺入蛋白质或转化为其他生物分子的细胞程序。 有趣的是，我们发现抑制这种氨基酸传感器也可以改变细胞处理碳水化合物的方式，如葡萄糖。我们的研究旨在确定细胞如何整合这些基本代谢物的利用，并定义营养队列影响细胞通信程序以维持代谢平衡的新机制。我们希望这些研究将为代谢的重要调控机制提供新的见解，并开发针对细胞代谢变化的新方法。