Oil and lipid improvements in field pea, a non-traditional oilseed crop

非传统油籽作物豌豆的油和脂质改良

• 批准号: 477365-2014
• 负责人: Lefsrud, Mark
• 金额: $ 2.33万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657105
• 关键词: Oil; lipid; improvements; field; pea

This research proposal aims to accelerate the development of an oil seed pea through exploration of natural variation and genetic enhancement of fatty acid accumulation in field pea. Our laboratories have recently studied molecular and biochemical aspects of lipid improvement in field pea. We have explored extraction, profile and accumulation of fatty acids including potential molecular markers associated with lipid content in pea. This needs further validation through analysis of a larger field pea population which will enable collection of a pea community for their use in molecular breeding. The improvement of the lipid content and fatty acid composition in field pea will then move toward the introduction of novel constructs for the genetic transformation of field pea to further understand lipid biosynthesis and accumulation. This research will result in the generation of a reproducible method for pea transformation through biolistic approach. The long term goal is to establish novel cultivars of field pea suitable for growth under a range of Canadian climatic conditions and to develop the infrastructure required to handle and process these oilseed peas.

本课题旨在通过探索大田豌豆脂肪酸积累的自然变异和遗传增强，加速油籽豌豆的开发。我们的实验室最近对大田豌豆脂质改善的分子和生化方面进行了研究。我们探索了豌豆中脂肪酸的提取、分布和积累，包括与脂质含量相关的潜在分子标记。这需要通过更大的大田豌豆群体分析进一步验证，以便收集豌豆群落用于分子育种。提高大田豌豆的脂质含量和脂肪酸组成将有助于引入新的构建体进行大田豌豆的遗传转化，从而进一步了解油脂的生物合成和积累。本研究将产生一种可复制的方法，豌豆转化通过生物学途径。长期目标是建立适合在加拿大一系列气候条件下生长的新型大田豌豆品种，并开发处理和加工这些油籽豌豆所需的基础设施。