The physiological role, structure, and regulation of animal alternative oxidases
动物替代氧化酶的生理作用、结构和调节
基本信息
- 批准号:RGPIN-2016-06135
- 负责人:
- 金额:$ 2.04万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2018
- 资助国家:加拿大
- 起止时间:2018-01-01 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
All organisms use electron transport systems (ETS) to generate energy for growth and reproduction. Eukaryotic organisms use ETS in mitochondria to produce ATP and the components of these ETS are highly variable in terms of the complexes that put electrons into the chain and the complexes that pull electrons out. In addition to the typical terminal oxidase in ETS, cytochrome c oxidase (COX), many organisms contain a second terminal oxidase called alternative oxidase (AOX). While AOX also utilizes oxygen as the final electron carrier and produces water, in contrast to COX, AOX does not pump protons and does not contribute to the proton motive force and proton gradient used to generate ATP. While at first glance this reaction appears to be energetically wasteful, AOX mRNA and protein levels greatly increase during environmental stress in many organisms. This implies that AOX plays a role in the successful acclimation to stress conditions, perhaps by conferring flexibility to the ETS.****AOX was initially discovered in plants and therefore most research has been conducted in this kingdom. AOX was long thought to be absent from animals, but work within the past decade has confirmed that it is present in some animals. We have a poor understanding of the taxonomic distribution of AOX in animals and know little about the in vivo expression of AOX mRNA and how it is regulated. Furthermore, little is known about AOX protein expression and post-translational regulation in animals.****We propose to identify new AOX sequences in animals using a combination of bioinformatics and molecular biology. This will provide us with the data needed to better understand the evolutionary history of the enzyme. We will also be characterizing the biochemical inhibitors and activators of AOX from Crassostrea gigas (Pacific oyster) by using high-resolution respirometry in order to better understand the post-translational regulation of the animal enzyme. Finally we will be investigating the physiological role of AOX in the stress tolerance of the intertidal copepod Tigriopus californicus. We are proposing to use T. californicus as a means of studying AOX mRNA and protein expression and regulation using laboratory experiments that manipulate the environmental conditions that the animal experiences. Our work will be the first complete molecular and physiological characterization of an animal AOX and will provide a means of comparison for researchers working in other organisms.***
所有生物都使用电子传递系统(ETS)来产生能量,以供生长和繁殖。真核生物利用线粒体中的ETS产生ATP这些ETS的成分在将电子放入链的复合物和将电子抽出的复合物方面是高度可变的。除了典型的末端氧化酶细胞色素c氧化酶(COX)外,许多生物体还含有第二种末端氧化酶,称为替代氧化酶(AOX)。虽然AOX也利用氧作为最终的电子载体并产生水,但与COX不同的是,AOX不泵送质子,也不参与产生ATP的质子动力和质子梯度。虽然乍一看这种反应似乎是能量浪费,但在许多生物体中,AOX mRNA和蛋白质水平在环境胁迫下大大增加。这意味着AOX在成功适应压力条件中发挥了作用,可能是通过赋予ETS灵活性。****AOX最初是在植物中发现的,因此大多数研究都是在这个王国进行的。长期以来,人们一直认为动物体内不存在AOX,但过去十年的研究证实,某些动物体内确实存在AOX。我们对AOX在动物中的分类学分布了解甚少,对AOX mRNA在体内的表达及其调控机制了解甚少。此外,AOX蛋白在动物中的表达和翻译后调控尚不清楚。****我们建议结合生物信息学和分子生物学在动物中鉴定新的AOX序列。这将为我们提供更好地了解这种酶的进化史所需的数据。为了更好地了解动物酶的翻译后调控,我们还将使用高分辨率呼吸测量法来表征长牡蛎(太平洋牡蛎)中AOX的生化抑制剂和激活剂。最后,我们将研究AOX在潮间带桡足动物加利福尼亚虎的抗逆性中的生理作用。我们建议利用加利福尼亚加利福尼亚鼠作为研究AOX mRNA和蛋白质表达和调控的手段,利用实验室实验来操纵动物所经历的环境条件。我们的工作将是动物AOX的第一个完整的分子和生理表征,并将为研究其他生物的研究人员提供一种比较手段
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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McDonald, Allison其他文献
Ethical Practices for Security Research with At-Risk Populations
高危人群安全研究的道德实践
- DOI:
10.1109/eurospw55150.2022.00065 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
Bhalerao, Rasika;Hamilton, Vaughn;McDonald, Allison;Redmiles, Elissa M.;Strohmayer, Angelika - 通讯作者:
Strohmayer, Angelika
403 Forbidden: A Global View of Geoblocking
403 Forbidden:地理封锁的全球视角
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
McDonald, Allison;Bernhard, Matthew;Valenta, Luke;VanderSloot, Benjamin;Scott, Will;Sullivan, Nick;Halderman, J Alex;Ensafi, Roya - 通讯作者:
Ensafi, Roya
Quack: Scalable Remote Measurement of Application-Layer Censorship.
Quack:应用层审查的可扩展远程测量。
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
VanderSloot, Benjamin;McDonald, Allison;Scott, Will;Halderman, J Alex;Ensafi, Roya - 通讯作者:
Ensafi, Roya
The Role of Computer Security Customer Support in Helping Survivors of Intimate Partner Violence
计算机安全客户支持在帮助亲密伴侣暴力幸存者中的作用
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
Zou, Yixin;McDonald, Allison;Narakornpichit, Julia;Dell, Nicola;Ristenpart, Thomas;Roundy, Kevin;Schaub, Florian;Tamersoy, Acar - 通讯作者:
Tamersoy, Acar
Recurrent subacute post-viral onset of ataxia associated with a PRF1 mutation
- DOI:
10.1038/ejhg.2013.20 - 发表时间:
2013-11-01 - 期刊:
- 影响因子:5.2
- 作者:
Dias, Cristina;McDonald, Allison;Boerkoel, Cornelius F. - 通讯作者:
Boerkoel, Cornelius F.
McDonald, Allison的其他文献
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{{ truncateString('McDonald, Allison', 18)}}的其他基金
Alternative oxidases in animals
动物体内的替代氧化酶
- 批准号:
RGPIN-2022-03570 - 财政年份:2022
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
The physiological role, structure, and regulation of animal alternative oxidases
动物替代氧化酶的生理作用、结构和调节
- 批准号:
RGPIN-2016-06135 - 财政年份:2021
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
The physiological role, structure, and regulation of animal alternative oxidases
动物替代氧化酶的生理作用、结构和调节
- 批准号:
RGPIN-2016-06135 - 财政年份:2020
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Science writing internship
科学写作实习
- 批准号:
555798-2020 - 财政年份:2020
- 资助金额:
$ 2.04万 - 项目类别:
Science Communication Skills Grant
The physiological role, structure, and regulation of animal alternative oxidases
动物替代氧化酶的生理作用、结构和调节
- 批准号:
RGPIN-2016-06135 - 财政年份:2017
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
The physiological role, structure, and regulation of animal alternative oxidases
动物替代氧化酶的生理作用、结构和调节
- 批准号:
RGPIN-2016-06135 - 财政年份:2016
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Molecular, regulatory. and functional properties of alternative oxidases of non-angiosperm plants
分子, 调节.
- 批准号:
402551-2011 - 财政年份:2015
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Molecular, regulatory. and functional properties of alternative oxidases of non-angiosperm plants
分子, 调节.
- 批准号:
402551-2011 - 财政年份:2014
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Molecular, regulatory. and functional properties of alternative oxidases of non-angiosperm plants
分子, 调节.
- 批准号:
402551-2011 - 财政年份:2013
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
Molecular, regulatory. and functional properties of alternative oxidases of non-angiosperm plants
分子, 调节.
- 批准号:
402551-2011 - 财政年份:2012
- 资助金额:
$ 2.04万 - 项目类别:
Discovery Grants Program - Individual
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