Refrigerated microcentrifuge for radioisotope samples in life sciences research

生命科学研究中放射性同位素样品的冷冻微量离心机

• 批准号: RTI-2019-00769
• 负责人: Salavati, Reza
• 金额: $ 1.2万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=664123
• 关键词: Refrigerated; microcentrifuge; radioisotope; samples; life

***We propose to purchase a refrigerated microcentrifuge for the Institute of Parasitology at McGill University. The instrument will be part of a new facility dedicated to radioisotope work for analytical methods and biological applications. It will be located at the Institute of Parasitology for convenient and safe access that will contain the experiments involving radioisotope labeling of various biological samples. It will support several research groups at the Institute of Parasitology who will use radioisotopes for sensitive analytical methods that will require controlled constant temperature. Also, more than 50 graduate student trainees per year in both thesis and non-thesis graduate programs administered by the Institute of Parasitology in the field of biotechnology can benefit from this facility and equipment. Therefore, significant usage is anticipated that will be managed and directed by Dr. R. Salavati. The refrigerated microcentrifuge will be used to process and prepare macromolecules including nucleic acids (RNA and DNA), proteins, and small chemical compounds that are labeled by a radioisotope (i.e., 32 P, 35S, 3H,) for increased sensitivity and subsequent use in a variety of enzymatic and binding assays. The controlled temperature during sample preparation for such assays is essential in maintaining the integrity of macromolecules as well as small chemical compounds to remain fully active. Therefore, the availability of this instrument will be essential to support the research program(s) and significantly facilitate both research abilities and a multidisciplinary training environment.

* 我们建议为麦吉尔大学寄生虫学研究所购买一台冷冻微量离心机。该仪器将是一个新设施的一部分，专门用于放射性同位素的分析方法和生物应用。它将设在寄生虫学研究所，方便和安全地进入，其中将包含涉及各种生物样品的放射性同位素标记的实验。它将支持寄生虫学研究所的几个研究小组，这些研究小组将使用放射性同位素进行需要控制恒温的敏感分析方法。此外，每年有50多名研究生学员在生物技术领域的寄生虫学研究所管理的论文和非论文研究生课程中受益于这一设施和设备。因此，预计将由R博士管理和指导大量使用。萨拉瓦蒂冷冻微量离心机将用于处理和制备大分子，包括核酸（RNA和DNA）、蛋白质和由放射性同位素标记的小化合物（即，32 P，35 S，3H），以提高灵敏度，并随后用于各种酶和结合测定。在用于此类测定的样品制备过程中，控制温度对于保持大分子的完整性以及小化合物保持完全活性至关重要。因此，该仪器的可用性将是必不可少的，以支持研究计划（S），并显着促进研究能力和多学科的培训环境。