Développement de biocatalyseurs novateurs de laccase et de tyrosinase pour l'élimination des polluants émergents dans les eaux usées

漆酶和酪氨酸酶生物催化剂创新者的开发，以消除水中污染物

• 批准号: 156894-2013
• 负责人: Jones, Peter
• 金额: $ 1.53万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=677831
• 关键词: veloppement; de; biocatalyseurs; novateurs; laccase

In this grant application, we propose to develop effective techniques to eliminate emerging pollutants including compounds which are endocrine disruptors. We will continue the development of aggregated enzymes especially combi-CLEAs which include two or more enzymes. The work proposed will use enzymes agglomerated as CLEA's in novel reactors which will include membrane bioreactors. The CLEAs will initially be of single enzymes such as laccase. The CLEAs for the membrane bioreactor will be CLEA's of more than one enzyme. These combi-CLEAs will allow the treatments of real wastewater where temperature, pH, and the presence of other compounds would otherwise make the enzymes either ineffective or at least force them to operate in less than optimal conditions. Target compounds for treatment will include bisphenol A, triclosan and nonylphenol. The membrane bioreactor should be able to operate at high flow rates, low pollutant concentrations with little loss of enzymes. This project will have as its outcome effective treatment for phenolic compounds using enzymes. Moreover the treatment will be practicable in the sense that it will be possible to undertake such treatment at a reasonable cost.

在这项拨款申请中，我们建议开发有效的技术来消除新出现的污染物，包括内分泌干扰物。 我们将继续开发聚合酶，特别是包含两种或多种酶的组合CLEA。拟议的工作将在新型反应器（包括膜生物反应器）中使用聚集为CLEA的酶。CLEA最初是单一酶，如漆酶。膜生物反应器的CLEA将是一种以上酶的CLEA。这些组合CLEA将允许处理真实的废水，其中温度、pH和其他化合物的存在将使酶无效或至少迫使它们在低于最佳条件下操作。治疗的目标化合物包括双酚A、三氯生和壬基酚。膜生物反应器应该能够在高流速、低污染物浓度下操作，并且酶的损失很少。该项目的成果将是使用酶对酚类化合物进行有效处理。 此外，处理将是切实可行的，即有可能以合理的费用进行这种处理。