Transcription factor stoichiometry defines distinct pluripotent states

转录因子化学计量定义了不同的多能状态

• 批准号: RGPIN-2016-05847
• 负责人: Hussein, Samer
• 金额: $ 2.62万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=686847
• 关键词: Transcription; factor; stoichiometry; defines; distinct

During human development, cells behave like building blocks and fascinatingly come together in an organized and well-controlled manner to build the human body. Within this process, we can think of cells as building blocks consisting of support foundations made up of complex protein interactions. These support foundations determine the choices of whether a cell becomes a blood, brain, or skin cell, or many other cell types that make up a human being. The earliest cell type that can be isolated, grown and studied is an embryonic stem cell. Interestingly, we can now convert adult cell into cells that highly resemble embryonic stem cells. This process, which is called “reprogramming”, requires the addition of certain factors. Increasing the levels of these factors gives rise to a unique cell type that resembles embryonic stem cells only in terms of differentiation potential. However, the two cell types completely differ in protein interactions that support the identity of embryonic stem cells. The proposed research aims on understanding the parallels between these two cell types. It also aims to identify new mechanisms of how altered protein levels lead to differences in protein interactions that can affect early embryo development. Specifically, we aim to resolve the important elements behind building a normal embryonic cell, which ultimately leads to normal development. We believe that our work will provide novel insights into the decisions taken by early cells of our body leading towards healthy human embryo development.**

在人类发展过程中，细胞的行为就像建立块，并以有组织且控制良好的方式迷人地融合在一起，以建立人体。在此过程中，我们可以将细胞视为由由复杂蛋白质相互作用组成的支持基础组成的构件。这些支持基础决定了细胞是否成为血液，大脑或皮肤细胞或许多其他构成人类的细胞类型的选择。可以分离，生长和研究的最早的细胞类型是胚胎干细胞。有趣的是，我们现在可以将成年细胞转化为高度相似的胚胎干细胞的细胞。这个过程称为“重编程”，需要添加某些因素。这些因素的水平提高导致独特的细胞类型，该细胞类型仅在分化潜力方面与胚胎干细胞类似。但是，两种细胞类型在蛋白质相互作用中完全不同，这些相互作用支持胚胎干细胞的身份。拟议的研究旨在了解这两种细胞类型之间的相似之处。它还旨在确定改变蛋白质水平改变的新机制，导致蛋白质相互作用的差异，这些蛋白质相互作用可能影响早期胚胎发育。具体而言，我们旨在解决构建正常胚胎细胞的重要元素，最终导致正常发育。我们认为，我们的工作将为您身体早期细胞做出的决定提供新的见解，从而导致健康的人类胚胎发育。**