Orthogonal RNA imaging and simple RNA detection tools

正交 RNA 成像和简单的 RNA 检测工具

• 批准号: 526365-2018
• 负责人: Unrau, Peter
• 金额: $ 2.91万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=687930
• 关键词: Orthogonal; RNA; imaging; simple; detection

International interest in the regulatory role of RNA is rapidly growing. Genomic regions previously thought of as 'junk' DNA play complex roles in gene regulation and are typically transcribed into RNA. Stress granules and other dynamic cellular particles contain RNA and are being found to play important roles in gene regulation. Tools for the study of RNA however are still embryonic and the global market for RNA research tools (excluding RNA interference) is anticipated to reach nearly 1 billion US dollars by 2020 (BCC research). While the study of proteins was revolutionized by the development of multicolour fluorescent protein tags and orthogonal protein based affinity tags, RNA imaging and purification methods have not kept pace. The first generation of RNA Mango, holds much potential for RNA imaging and RNA protein complex purification, but currently does not allow multichannel imaging or orthogonal purifications. This NSERC CRD proposal aims to directly address these issues so as to continue the rapid commercialization of this Canadian technology together with Applied Biological Materials (Burnaby, BC).The proposal has two specific aims: The first is to develop an orthogonal RNA Mango system capable of imaging two RNAs simultaneously. These new high affinity aptamers will allow the development of two colour imaging and orthogonal purification methodologies and will have many in vitro and in vivo applications. The second aim of this proposal will apply first generation Mango technology to the detect of low concentrations of RNA using isothermal methods. Together this proposal will generate a broad range of commercializable elements that will significantly extend the utility of first generation RNA Mango technology.

国际上对RNA的调节作用的兴趣正在迅速增长。以前被认为是“垃圾”DNA的基因组区域在基因调控中扮演着复杂的角色，通常被转录成RNA。应激颗粒和其他动态细胞颗粒含有RNA，并被发现在基因调控中发挥重要作用。然而，研究RNA的工具仍处于萌芽阶段，RNA研究工具(不包括RNA干扰)的全球市场预计到2020年将达到近10亿美元(BCC研究)。虽然多色荧光蛋白质标签和基于蛋白质的正交亲和标签的发展给蛋白质的研究带来了革命性的变化，但RNA成像和纯化方法并没有跟上步伐。第一代RNA芒果，在RNA成像和RNA蛋白复合体纯化方面具有很大的潜力，但目前不允许多通道成像或正交纯化。NSERC CRD计划旨在直接解决这些问题，以便继续将这项加拿大技术与应用生物材料公司(BC)一起快速商业化。该计划有两个具体目标：第一个目标是开发能够同时对两个RNA进行成像的正交RNA芒果系统。这些新的高亲和力适配子将允许开发两种彩色成像和正交纯化方法，并将在体外和体内有许多应用。这项提议的第二个目标是将第一代芒果技术应用于使用等温法检测低浓度的RNA。这一提议将产生一系列可商业化的元素，这些元素将显著扩展第一代RNA芒果技术的用途。