Structure and Function of Bacterial Nanowires

细菌纳米线的结构和功能

基本信息

  • 批准号:
    RGPIN-2020-04837
  • 负责人:
  • 金额:
    $ 2.99万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2020
  • 资助国家:
    加拿大
  • 起止时间:
    2020-01-01 至 2021-12-31
  • 项目状态:
    已结题

项目摘要

Bacterial nanowires are naturally occurring electrically conductive extensions produced by some anaerobic bacteria, including Geobacter sulfurreducens. They are primarily used to find electron acceptors in the environment so the bacteria can shed excess electrons produced during respiration, which is particularly relevant when oxygen is not available. These same bacterial strains can also form biofilms, and in these biofilms the nanowires link into a large network of nanowires. Networks can in some cases include nanowires from bacteria of a different species, which increases the likelihood of finding an electron acceptor. This network of natural electron-emitting bacterial nanowires has great potential for use as a microbial fuel cells, where we can gain electrical energy directly from the organisms. The goal of the proposed research is to elucidate the mechanism of electrical conduction through bacterial nanowires in biofilms. Our recent publication on a previously unknown cytochrome-based nanowire (OmcS) demonstrates how much is to be learned about the molecular mechanisms of conduction in protein filaments. Key questions we will address include: How many different types of conductive filaments exist? How is electricity conducted along a pilus? Is the regular array within the pilus disrupted when a neighbouring pilus makes contact, and if so, how is conductivity maintained? Do biofilms produce thick cables of conductive nanowires, or is the network delocalised? We will study nanowires from G. sulfurreducens as a representative system of bacterial nanowires using cryoelectron microscopy and single particle analysis for isolated nanowires, as well as focused ion beam (FIB)-milled biofilms and electron tomography for in situ studies of biofilms. This work will help us understand how bacteria work together in a network, and what limitations are inherent to the function of a biofilm. We expect that with some modifications to the structure of the nanowires, we can improve efficiency of electric conduction, leading to the design of an improved microbial fuel cell. Additionally, this research will help establish an important scientific method in Canada for the first time, in situ imaging of cells and cellular networks with molecular detail. The combination of FIB milling and cryo-electron tomography is proving to be very powerful, and its introduction into the Canadian research community is likely to have a strong impact and augment the work of many groups across the country.
细菌纳米线是由一些厌氧细菌(包括硫还原地杆菌)产生的自然存在的导电延伸。它们主要用于在环境中寻找电子受体,这样细菌就可以排出呼吸过程中产生的多余电子,这在没有氧气的情况下尤为重要。这些细菌菌株也可以形成生物膜,在这些生物膜中,纳米线连接成一个巨大的纳米线网络。在某些情况下,网络可以包括来自不同种类细菌的纳米线,这增加了找到电子受体的可能性。这种天然的电子发射细菌纳米线网络在用作微生物燃料电池方面具有很大的潜力,我们可以直接从生物那里获得电能。

项目成果

期刊论文数量(0)
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Strauss, Mike其他文献

Stabilising cubosomes with Tween 80 as a step towards targeting lipid nanocarriers to the blood-brain barrier
Covalently circularized nanodiscs for studying membrane proteins and viral entry.
  • DOI:
    10.1038/nmeth.4079
  • 发表时间:
    2017-01
  • 期刊:
  • 影响因子:
    48
  • 作者:
    Nasr, Mahmoud L.;Baptista, Diego;Strauss, Mike;Sun, Zhen-Yu J.;Grigoriu, Simina;Huser, Sonja;Plueckthun, Andreas;Hagn, Franz;Walz, Thomas;Hogle, James M.;Wagner, Gerhard
  • 通讯作者:
    Wagner, Gerhard
Open and closed domains in the mouse genome are configured as 10-nm chromatin fibres
  • DOI:
    10.1038/embor.2012.139
  • 发表时间:
    2012-11-01
  • 期刊:
  • 影响因子:
    7.7
  • 作者:
    Fussner, Eden;Strauss, Mike;Bazett-Jones, David P.
  • 通讯作者:
    Bazett-Jones, David P.
RNA Transfer from Poliovirus 135S Particles across Membranes Is Mediated by Long Umbilical Connectors
  • DOI:
    10.1128/jvi.03209-12
  • 发表时间:
    2013-04-01
  • 期刊:
  • 影响因子:
    5.4
  • 作者:
    Strauss, Mike;Levy, Hazel C.;Hogle, James M.
  • 通讯作者:
    Hogle, James M.
Structure and function of a hexameric cyanophycin synthetase 2.
  • DOI:
    10.1002/pro.4685
  • 发表时间:
    2023-07
  • 期刊:
  • 影响因子:
    8
  • 作者:
    Markus, Linda M. D.;Sharon, Itai;Munro, Kim;Grogg, Marcel;Hilvert, Donald;Strauss, Mike;Schmeing, T. Martin
  • 通讯作者:
    Schmeing, T. Martin

Strauss, Mike的其他文献

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{{ truncateString('Strauss, Mike', 18)}}的其他基金

Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
  • 批准号:
    RGPAS-2020-00011
  • 财政年份:
    2022
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Discovery Grants Program - Accelerator Supplements
Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
  • 批准号:
    RGPIN-2020-04837
  • 财政年份:
    2022
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Discovery Grants Program - Individual
Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
  • 批准号:
    RGPIN-2020-04837
  • 财政年份:
    2021
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Discovery Grants Program - Individual
Cryo-3D-super-resolution light microscope for correlative microscopy
用于相关显微镜的 Cryo-3D 超分辨率光学显微镜
  • 批准号:
    RTI-2022-00350
  • 财政年份:
    2021
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Research Tools and Instruments
Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
  • 批准号:
    RGPAS-2020-00011
  • 财政年份:
    2021
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Discovery Grants Program - Accelerator Supplements
Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
  • 批准号:
    RGPAS-2020-00011
  • 财政年份:
    2020
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Discovery Grants Program - Accelerator Supplements
Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
  • 批准号:
    DGECR-2020-00018
  • 财政年份:
    2020
  • 资助金额:
    $ 2.99万
  • 项目类别:
    Discovery Launch Supplement

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Structure and Function of Bacterial Nanowires
细菌纳米线的结构和功能
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